Passa al contenuto
Merck

ECM541

Sigma-Aldrich

ECM Cell Culture Optimization Array (Colorimetric, 96-Wells)

Slide 1 of 1
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali

Scegli un formato

1 KIT
723,00 €

723,00 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.

Scegli un formato

Cambia visualizzazione
1 KIT
723,00 €

About This Item

Codice UNSPSC:
12352207
eCl@ss:
32161000
NACRES:
NA.32

Documenti fondamentali

Visualizza tutta la documentazione

Vai a

Servizio Tecnico
Hai bisogno di aiuto? Il nostro team di scienziati qualificati è a tua disposizione.
Permettici di aiutarti

723,00 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Servizio Tecnico
Hai bisogno di aiuto? Il nostro team di scienziati qualificati è a tua disposizione.
Permettici di aiutarti

tecniche

cell based assay: suitable

Livello qualitativo

100

input

sample type: human embryonic stem cell(s)
sample type epithelial cells
sample type mesenchymal stem cell(s)
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type hematopoietic stem cell(s)

Metodo di rivelazione

colorimetric

Confronta articoli simili

Visualizza il confronto per intero

Mostra le differenze

1 of 4

Questo articolo
ECM540ECM545ECM531
ECM Cell Culture Optimization Array (Colorimetric, 96-Wells)

ECM541

ECM Cell Culture Optimization Array (Colorimetric, 96-Wells)

ECM Cell Adhesion Array Kit, colorimetric The ECM Cell Adhesion Array kit is useful for assessing specific cell surface integrins, monitoring in vitro cell differentiation, or screening potential cell adhesion promoters/inhibitors.

ECM540

ECM Cell Adhesion Array Kit, colorimetric

Kit per la valutazione dell’adesione cellulare su array rivestiti con ECM, metodo fluorimetrico The ECM Cell Adhesion Array kit is useful for assessing specific cell surface integrins, monitoring in vitro cell differentiation, or screening potential cell adhesion promoters/inhibitors.

ECM545

Kit per la valutazione dell′adesione cellulare su array rivestiti con ECM, metodo fluorimetrico

β Integrin-mediated Cell Adhesion Array Kit, colorimetric This assay can substitute for FACS analysis & is useful for screening human adherent cells for cell surface integrins or for monitoring in vitro cell differentiation or genetic modification of cells.

ECM531

β Integrin-mediated Cell Adhesion Array Kit, colorimetric

technique(s)

cell based assay: suitable

technique(s)

cell based assay: suitable

technique(s)

cell based assay: suitable

technique(s)

cell based assay: suitable

input

sample type: human embryonic stem cell(s)
sample type epithelial cells
sample type mesenchymal stem cell(s)
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type hematopoietic stem cell(s)

input

-

input

-

input

-

detection method

colorimetric

detection method

colorimetric

detection method

fluorometric

detection method

colorimetric

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

Descrizione generale

Extracellular matrix (ECM) proteins are produced intracellularly and are subsequently secreted into the surrounding cellular medium, actively regulating a diverse range of cell functions including cell adhesion, differentiation, proliferation, migration, invasion and survival. A primary utility of ECMs in in vitro culture is to promote cellular adhesion while maintaining cell viability and maximizing cell proliferation for downstream cell-based applications. In cases where optimal cell growth conditions are not well defined and critical adhesion protein requirements are unknown, identifying the ideal ECM protein(s) at functionally relevant concentration(s) can be a time-consuming and labor-intensive process. The 96-well, colorimetric ECM Cell Culture Optimization Array provides a multiparametric assay that allows researchers to: (1) quickly identify ECM(s) that best promote cell-type specific adhesion and (2) pinpoint the optimal ECM concentration required to promote maximum adhesion for a particular cell type.

How does the ECM Cell Culture Optimization Array work?
The foundation of our kit is a 96-well microtiter plate containing several commonly-used ECM proteins. Collagen I, laminin, fibronectin, and vitronectin are arrayed in triplicate with concentrations ranging from 0.125 μg/mL to 20 μg/mL. You simply culture your cells on the plate, and use the included reagents to block, stain, and analyze the adhesion capacity with a plate reader. We have extensively tested and optimized this kit on a variety of cell types including HEK293 cells, and human and rodent neural stem cells.

Applicazioni

Research Category
Cell Structure
The ECM Cell Culture Optimization Array is the first commercially-available tool of its kind to enable researchers to not only quickly identify the best ECM protein for their cell culture environment, but also pinpoint the concentration needed to achieve optimal cell growth conditions.
The ECM cell culture Optimization Array enables researchers to identify the best ECM protein for their cell culture environment & also pinpoint the concentration needed to achieve optimal cell growth conditions.

Confezionamento

1 plate, 96-wells

Nota sulla preparazione

The ECM Gradient Array Plate should be stored in the foil pouch at -70ºC, and used within three months of the date of receipt. The remaining kit components should be stored at 2º to 8ºC.

Altre note

  • ECM Gradient Array Plate: One 96-well plate with 12 strips
  • 30%BSA: (Part No. CS201638) One vial - 0.5mL
  • Cell Stain Solution: (Part No. 90144) One bottle - 20 mL.
  • Extraction Buffer: (Part No. 90145) One bottle - 20 mL
  • Assay Buffer: (Part No. 2003646) One bottle - 100 mL

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Pittogrammi

FlameExclamation mark
GHS02,GHS07

Avvertenze

Danger

Indicazioni di pericolo

H225,H319

Classi di pericolo

Eye Irrit. 2 - Flam. Liq. 2

Codice della classe di stoccaggio

3 - Flammable liquids

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Domande

Recensioni

Nessuna valutazione

Filtri attivi

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica