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324708 Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi

324708
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Panoramica

Replacement Information

Tabella delle specifiche principali

Products

Numero di catalogoConfezionamento Qtà/conf
324708-2UG Fiala di plastica 2 μg
Description
OverviewNative endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
Catalogue Number324708
Brand Family Calbiochem®
References
ReferencesDrapeau, G.R. 1980. J. Biol. Chem. 255, 839.
Product Information
CAS number9001-92-7
Unit of DefinitionOne unit is defined as the amount of enzyme that will release a sufficient amount of azo dye from AZOCOLL™ Substrate (Cat. No. 194932) to produce an increase in absorbance at 520 nm of 0.001 per min at 37°C, pH 7.5.
EC number3.4.24.33
FormWhite lyophilized solid
FormulationLyophilized from 10 mM Tris-HCl, pH 7.5.
Quality LevelMQ100
Applications
Biological Information
Purity≥90% by SDS-PAGE
Specific Activity≥20,000 units/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 20-36/37/38-42/43

Harmful by inhalation.
Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation and skin contact.
S PhraseS: 22-26-36

Do not breathe dust.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi MSDS

Titolo

Scheda di sicurezza (MSDS) 

Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi Certificati d'Analisi

TitoloNumero di lotto
324708

Riferimenti bibliografici

Panoramica delle referenze
Drapeau, G.R. 1980. J. Biol. Chem. 255, 839.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-June-2008 RFH
DescriptionNative endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins and the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK.
FormWhite lyophilized solid
FormulationLyophilized from 10 mM Tris-HCl, pH 7.5.
Recommended reaction conditions
Protocol 1. Reconstitute in 50 µl of distilled H2O. 2. Dissolve the proteins to be sequenced in phosphate, acetate, or Tris buffer, pH 6.0-8.5. For proteins that are difficult to solubilize, add urea (up to 1 M), SDS (0.01%), or guanidine-HCl (up to 1 M) to the digestion buffer. 3. Use a 1:20-1:100 ratio of proteinase to protein by weight for sequence analysis. 4. Incubate between 2-18 h at 37°C. Incubation time is dependent on the amount of enzyme used.
CAS number9001-92-7
EC number3.4.24.33
Purity≥90% by SDS-PAGE
Specific activity≥20,000 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will release a sufficient amount of azo dye from AZOCOLL™ Substrate (Cat. No. 194932) to produce an increase in absorbance at 520 nm of 0.001 per min at 37°C, pH 7.5.
SolubilityH₂O
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
Toxicity Harmful
ReferencesDrapeau, G.R. 1980. J. Biol. Chem. 255, 839.

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Categorie

Life Science Research > Proteins and Enzymes > Other Enzymes