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324845 Endothelial Cell Growth Supplement, Bovine Neural Tissue

324845
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Products

Numero di catalogoConfezionamento Qtà/conf
324845-15MG Fiala di plastica 15 mg
Description
OverviewNative endothelial cell growth supplement from bovine neural tissue. A cell culture-medium supplement screened for contamination by bacteria, fungi, and mycoplasma. Mitogenic activity thought to be physically, chemically, and biologically distinct from that of FGF. Acts as a superior cloning factor for the isolation and growth of hybridoma cells and may eliminate the need for feeder cell layers.
Catalogue Number324845
Brand Family Calbiochem®
SynonymsECGS
References
ReferencesBlann, A.D. 1991. Cell Biol. Int. Rep. 15, 493.
Jassal, D., et al. 1991. In Vitro Cell. Dev. Biol. 27A, 625.
Tanswell, A.K., et al. 1991. J. Dev. Physiol. 15, 199.
Olander, J., et al. 1980. In Vitro 16, 209.
Product Information
FormLyophilized
FormulationLyophilized from sterile-filtered NaCl containing streptomycin sulfate.
Quality LevelMQ100
Applications
Biological Information
Biological activitygrowth-promoting activity confirmed with fetal bovine endothelial cells
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 20/21/22-61

Harmful by inhalation, in contact with skin and if swallowed.
May cause harm to the unborn child.
S PhraseS: 22-36/37/39-45

Do not breathe dust.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful & Carcinogenic / Teratogenic
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Endothelial Cell Growth Supplement, Bovine Neural Tissue Certificati d'Analisi

TitoloNumero di lotto
324845

Riferimenti bibliografici

Panoramica delle referenze
Blann, A.D. 1991. Cell Biol. Int. Rep. 15, 493.
Jassal, D., et al. 1991. In Vitro Cell. Dev. Biol. 27A, 625.
Tanswell, A.K., et al. 1991. J. Dev. Physiol. 15, 199.
Olander, J., et al. 1980. In Vitro 16, 209.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-June-2008 RFH
SynonymsECGS
DescriptionNative endothelial cell growth supplement from bovine neural tissue. A cell-culture-medium supplement for the growth and maintenance of many cell types. Growth promoting activities have been described for human endothelial cells, bovine endothelial cells, and mouse Balb/c fibroblasts. Can also substitute for feeder cells in support of the clonal growth of hybridomas. Mitogenic activity of the preparation is thought to be physically, chemically, and biologically distinct from that of FGF. Media containing 50-150 μg/ml is recommended for culturing vascular endothelial cells. Acts as a superior cloning factor for the isolation and growth of hybridoma cells and may eliminate the need for feeder cell layers. Screened for contamination by bacteria, fungi, and mycoplasma.
FormLyophilized
FormulationLyophilized from sterile-filtered NaCl containing streptomycin sulfate.
Concentration Label Please refer to vial label for lot-specific concentration
Biological activitygrowth-promoting activity confirmed with fetal bovine endothelial cells
SolubilityUsing aseptic technique, add 1-5 ml sterile serum-free medium and solubilize by gentle aspiration with a sterile pipette.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Toxicity Harmful & Carcinogenic / Teratogenic
ReferencesBlann, A.D. 1991. Cell Biol. Int. Rep. 15, 493.
Jassal, D., et al. 1991. In Vitro Cell. Dev. Biol. 27A, 625.
Tanswell, A.K., et al. 1991. J. Dev. Physiol. 15, 199.
Olander, J., et al. 1980. In Vitro 16, 209.