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374096 Eosinophil Peroxidase, Human

374096
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Products

Numero di catalogoConfezionamento Qtà/conf
374096-50UG Fiala di plastica 50 μg
Description
OverviewNative, human eosinophil peroxidase. Eosinophil peroxidase is a secreted hemoprotein that catalyzes oxidations of various substrates by hydrogen peroxide. Together with other leukocyte-derived enzymes, such as Myeloperoxidase (Cat. No. 475911), EPO plays an important role in the pathogenesis of asthma by mediating oxidative events that lead to the production of ROS (reactive oxygen species) and RNS (reactive nitrogen species).
Catalogue Number374096
Brand Family Calbiochem®
SynonymsEPO
References
ReferencesAndreadis., et al. 2003. Free Radic. Biol. Med. 35, 213.
Borelli, V., et al. 2003. Infect. Immun. 71, 605.
Erpenbeck, V.J., et al. 2003. Clin. Exp. Allergy 33, 331.
McElhinney, B., et al. 2003. J. Leukoc. Biol. 74, 897.
Aldridge, R.E., et al. 2002. Free Radic. Biol. Med. 33, 847.
Kettle, A.J., and Winterbourn, C.C. 1988. Biochim. Biophys. Acta 957, 185.
Desser, R.K., et al. 1972. Arch. Biochem. Biophys. 148, 452.
Hager, L.P., et al. 1966. J. Biol. Chem. 241, 1769.
Product Information
CAS number9003-99-0
Unit of DefinitionOne unit is defined as the amount of enzyme that will cause an increase in absorbance of 1 at 470 nm per min at pH 7.0 and 25°C, calculated from the initial linear rate of reaction using guaiacol as substrate.
EC number1.11.1.7
FormLyophilized
FormulationLyophilized from 5 mM phosphate buffer, pH 7.0.
Quality LevelMQ100
Applications
Biological Information
Purity≥90% by SDS-PAGE
SourcePrepared from tissue that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV and HIV.
Specific Activity≥ 270 units/mg protein (Guaiacol Assay Method)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C). If solutions must be frozen the protein concentration should be >1 mg/ml and the activity determined following each thaw.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Eosinophil Peroxidase, Human MSDS

Titolo

Scheda di sicurezza (MSDS) 

Eosinophil Peroxidase, Human Certificati d'Analisi

TitoloNumero di lotto
374096

Riferimenti bibliografici

Panoramica delle referenze
Andreadis., et al. 2003. Free Radic. Biol. Med. 35, 213.
Borelli, V., et al. 2003. Infect. Immun. 71, 605.
Erpenbeck, V.J., et al. 2003. Clin. Exp. Allergy 33, 331.
McElhinney, B., et al. 2003. J. Leukoc. Biol. 74, 897.
Aldridge, R.E., et al. 2002. Free Radic. Biol. Med. 33, 847.
Kettle, A.J., and Winterbourn, C.C. 1988. Biochim. Biophys. Acta 957, 185.
Desser, R.K., et al. 1972. Arch. Biochem. Biophys. 148, 452.
Hager, L.P., et al. 1966. J. Biol. Chem. 241, 1769.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-July-2008 RFH
SynonymsEPO
DescriptionNative, human eosinophil peroxidase. Eosinophil peroxidase is a secreted hemoprotein that catalyzes the oxidation of various substrates by hydrogen peroxide. Together with other leukocyte-derived enzymes, such as Myeloperoxidase (Cat. No. 475911), EPO plays an important role in the pathogenesis of asthma by mediating oxidative events that lead to the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS).
FormLyophilized
FormulationLyophilized from 5 mM phosphate buffer, pH 7.0.
SourcePrepared from tissue that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV and HIV.
CAS number9003-99-0
EC number1.11.1.7
Purity≥90% by SDS-PAGE
Specific activity≥ 270 units/mg protein (Guaiacol Assay Method)
Unit definitionOne unit is defined as the amount of enzyme that will cause an increase in absorbance of 1 at 470 nm per min at pH 7.0 and 25°C, calculated from the initial linear rate of reaction using guaiacol as substrate.
SolubilityReconstitute in 400 µl sterile, distilled H₂O to yield a final concentration of ~1.7 µM.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C). If solutions must be frozen the protein concentration should be >1 mg/ml and the activity determined following each thaw.
Toxicity Standard Handling
ReferencesAndreadis., et al. 2003. Free Radic. Biol. Med. 35, 213.
Borelli, V., et al. 2003. Infect. Immun. 71, 605.
Erpenbeck, V.J., et al. 2003. Clin. Exp. Allergy 33, 331.
McElhinney, B., et al. 2003. J. Leukoc. Biol. 74, 897.
Aldridge, R.E., et al. 2002. Free Radic. Biol. Med. 33, 847.
Kettle, A.J., and Winterbourn, C.C. 1988. Biochim. Biophys. Acta 957, 185.
Desser, R.K., et al. 1972. Arch. Biochem. Biophys. 148, 452.
Hager, L.P., et al. 1966. J. Biol. Chem. 241, 1769.