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341298 FASTsilver™ Gel Staining Kit

341298
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Products

Numero di catalogoConfezionamento Qtà/conf
341298-1KIT Bottiglia di vetro 1 kit
Description
OverviewA rapid and sensitive method for detecting protein and nucleic acids fractionated by PAGE. Provides gels with clear backgrounds and sharp bands. Silver staining is about 100 times more sensitive than the standard Coomassie Blue protein staining and about 10 times more sensitive than ethidium bromide for DNA and RNA.
Catalogue Number341298
Brand Family Calbiochem®
Materials Required but Not Delivered Ethanol
Glacial acetic acid
TCA
Ultra-pure water.
References
Product Information
Kit containsSilver stain, developer, sensitizers I and II, and a user protocol. Note: one kit provides reagents sufficient for staining 25 mini gels.
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Intended useFASTsilver™ is one of the most rapid and sensitive methods for detecting proteins and nucleic acids fractionated by PAGE. Staining is about 100 times more sensitive than Coomassie Blue protein staining and about 10 times more sensitive than ethidium bromide for DNA and RNA. FASTsilver™ provides gels with exceptionally clear backgrounds and sharp protein or nucleic acid images. The protocol is simple and takes as little as 60 min to yield perfect results.
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +15°C to +30°C
Storage ConditionsUpon arrival store entire kit contents at room temperature (20°C).
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Kit containsSilver stain, developer, sensitizers I and II, and a user protocol. Note: one kit provides reagents sufficient for staining 25 mini gels.
Specifications

Documentation

FASTsilver™ Gel Staining Kit MSDS

Titolo

Scheda di sicurezza (MSDS) 

FASTsilver™ Gel Staining Kit Certificati d'Analisi

TitoloNumero di lotto
341298
Protocollo per l'utilizzatore

Revision12-April-2011 RFH
StorageUpon arrival store entire kit contents at room temperature (20°C).
Intended useFASTsilver™ is one of the most rapid and sensitive methods for detecting proteins and nucleic acids fractionated by PAGE. Staining is about 100 times more sensitive than Coomassie Blue protein staining and about 10 times more sensitive than ethidium bromide for DNA and RNA. FASTsilver™ provides gels with exceptionally clear backgrounds and sharp protein or nucleic acid images. The protocol is simple and takes as little as 60 min to yield perfect results.
Materials provided(Suitable for 25 mini gels)

• Silver Stain (Kit component No. KP15701): 1 bottle, 125 ml
• Developer (Kit component No. KP15702): 1 vial, 75 g
• Sensitizer-I (Kit component No. KP15703): 1 vial, 4 ml
• Sensitizer-II (Kit component No. KP15704): 1 vial, 4 ml
Materials Required but not provided Ethanol
Glacial acetic acid
TCA
Ultra-pure water.
Reagent preparation• Fixative I: Mix 30 ml 100% ethanol, 10 ml glacial acetic acid, and 60 ml ultra-pure water to obtain 100 ml Fixative I. • Fixative II: Mix 10 ml 100% ethanol and 90 ml ultra-pure water to obtain 100 ml Fixative II. • Sensitizer: Mix 5 ml silver stain, 65 µl sensitizer-I, and 45 ml ultra-pure water to obtain 50 ml Sensitizer. • Developer: Mix 2.5 g developer, 32.5 µl sensitizer-I, 32.5 µl sensitizer-II, and 50 ml ultra-pure water to obtain 50 ml Developer. • Stopper: Mix 11 ml glacial acetic acid and 39 ml ultra-pure water to obtain 50 ml Stopper.
Detailed protocol1. After electrophoresis, fix the gel in 50 ml of the freshly prepared Fixative I. Use highly purified deionized water to make the solution. For isoelectric focusing gels, fix the gel first in 20% TCA for 60 min.

2. Fix the gel for 30 min to 3 h depending upon its thickness. For mini gels, (8 x 10 cm), 30 min is sufficient.

3. Wash twice, 10 min each, in 50 ml of the freshly prepared Fixative II.

4. Wash three times, 10 min each, in ultra-pure water. During washing Steps 2 and 3, use generous amounts of water (100 ml per 8 x 10 cm mini gels) and use gentle rocking or agitation.

5. Soak the gel in 50 ml of freshly prepared Sensitizer for 30 min, with gentle rocking of the gel, depending upon the thickness of the gel.

6. Rinse the gel only for 10-20 s with 50 ml of ultra-pure water.

7. Soak the gel in 50 ml of freshly prepared Developer. Gently rock the gel until bands are visible. Band intensity will develop quickly.

8. As soon as band intensity reaches an acceptable level, stop color development by adding 5 ml of the freshly prepared Stopper. Gently rock the gel for 10 min. Store the gel in ultra-pure water for subsequent analysis.
Sensitivity NotesThe lower detection limit of FASTsilver™ gel stain is 1 ng/band for proteins and nucleic acids.
Registered TrademarksCalbiochem® is a registered trademark of EMD Chemicals, Inc.
Interactive Pathways™ is a trademark of EMD Chemicals, Inc.
FASTsilver™ is a trademark of Geno Technology, Inc.