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371818 G-Protein βγ Binding Peptide, mSIRK - Calbiochem

371818
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Empirical Formula
C₉₃H₁₅₀N₂₀O₂₅

Products

Numero di catalogoConfezionamento Qtà/conf
371818-1MG Bottiglia di vetro 1 mg
371818-5MG Bottiglia di vetro 5 mg
Description
OverviewA cell-permeable, N-myristoylated G-protein βγ selective binding peptide that disrupts the interaction between α and βγ subunits and promotes the α subunit dissociation without stimulating nucleotide exchange. Shown to cause a rapid activation of ERK1/2 (EC50 ~ 2.5 - 5 µM) in rat arterial smooth muscle and Rat2 cells. Also promotes the phosphorylation of JNK and p38 MAP kinase, phospholipase C and causes Ca2+ release from internal stores. Negative control is also available (Cat. No. 371820).
Catalogue Number371818
Brand Family Calbiochem®
SynonymsmSIRK, myr-SIRKALNILGYPDYD-OH
References
ReferencesGoubaeva, F., et al. 2003. J. Biol. Chem. 278, 19634.
Scott, J.K., et al. 2001. EMBO J. 20, 767.
Product Information
ATP CompetitiveN
FormLyophilized
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₉₃H₁₅₀N₂₀O₂₅
Chemical formulaC₉₃H₁₅₀N₂₀O₂₅
Hygroscopic Hygroscopic
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetN-myristoylated G-protein βγ selective binding
Primary Target IC<sub>50</sub>EC50 ~ 2.5 - 5 µM in activation of ERK1/2 in rat arterial smooth muscle and Rat2 cells
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Peptide SequenceMyr-N-Ser-Ile-Arg-Lys-Ala-Leu-Asn-Ile-Leu-Gly-Tyr-Pro-Asp-Tyr-Asp-OH
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

G-Protein βγ Binding Peptide, mSIRK - Calbiochem MSDS

Titolo

Scheda di sicurezza (MSDS) 

G-Protein βγ Binding Peptide, mSIRK - Calbiochem Certificati d'Analisi

TitoloNumero di lotto
371818

Riferimenti bibliografici

Panoramica delle referenze
Goubaeva, F., et al. 2003. J. Biol. Chem. 278, 19634.
Scott, J.K., et al. 2001. EMBO J. 20, 767.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-July-2008 RFH
SynonymsmSIRK, myr-SIRKALNILGYPDYD-OH
DescriptionA cell-permeable, N-myristoylated G-protein βγ selective binding peptide that disrupts the interaction between α and βγ subunits and promotes α subunit dissociation without stimulating nucleotide exchange. Reported to cause a rapid activation of ERK1/2 (EC50 ~2.5-5 µM) in RASM (rat arterial smooth muscle) and Rat2 cells. Promotes the phosphorylation of JNK and p38 MAP kinase. Also increases phospholipase C activity and causes Ca2+ release from internal stores.
FormLyophilized
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₉₃H₁₅₀N₂₀O₂₅
Peptide SequenceMyr-N-Ser-Ile-Arg-Lys-Ala-Leu-Asn-Ile-Leu-Gly-Tyr-Pro-Asp-Tyr-Asp-OH
Purity≥95% by HPLC
SolubilityDMSO (5 mg/ml)
Storage +2°C to +8°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C
Toxicity Standard Handling
ReferencesGoubaeva, F., et al. 2003. J. Biol. Chem. 278, 19634.
Scott, J.K., et al. 2001. EMBO J. 20, 767.