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407615 Interleukin-1β, Human, Recombinant, E. coli

407615
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Products

Numero di catalogoConfezionamento Qtà/conf
407615-5UG Fiala di plastica 5 μg
Description
OverviewRecombinant, human interleukin-1β expressed in E. coli. Major form of interleukin-1 secreted by monocytes and macrophages. Induces nitric oxide synthase in pancreatic and smooth muscle cells and suppresses apoptosis in rat ovarian follicles. Involved in inflammatory and immune responses and promotes wound healing.
Catalogue Number407615
Brand Family Calbiochem®
SynonymsrhIL-1β
References
ReferencesChun, S.Y., et al. 1995. Endocrinology 136, 3120.
Kunz, D., et al. 1994. Biochem. J. 304, 337.
Eizirik, D.L., et al. 1993. FEBS Lett. 317, 62.
Dinarello, C>A> 1991. Blood 77, 1627.
Casagli, M.C., et al. 1989. Biochem. Biophys. Res. Commun. 162, 357.
Symons, J.A., et al. 1987. In Lymphokines and interferons, A Practical Approach (Clemens, M.J., et al. eds.) IRL Press, Oxford p. 272.
Product Information
CAS number97599-21-8
FormLyophilized
FormulationLyophilized from sterile-filtered PBS, 50 µg BSA/µg IL-1β.
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀ = <12 pg/ml as measured in a cell proliferation assay using the murine T-cell line, D10.G4.1 (Symons, J.A. et al. 1987)
Purity≥97% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤1.0 EU/µg IL-1β
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSNM9740000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Interleukin-1β, Human, Recombinant, E. coli MSDS

Titolo

Scheda di sicurezza (MSDS) 

Interleukin-1β, Human, Recombinant, E. coli Certificati d'Analisi

TitoloNumero di lotto
407615

Riferimenti bibliografici

Panoramica delle referenze
Chun, S.Y., et al. 1995. Endocrinology 136, 3120.
Kunz, D., et al. 1994. Biochem. J. 304, 337.
Eizirik, D.L., et al. 1993. FEBS Lett. 317, 62.
Dinarello, C>A> 1991. Blood 77, 1627.
Casagli, M.C., et al. 1989. Biochem. Biophys. Res. Commun. 162, 357.
Symons, J.A., et al. 1987. In Lymphokines and interferons, A Practical Approach (Clemens, M.J., et al. eds.) IRL Press, Oxford p. 272.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision13-May-2008 RFH
SynonymsrhIL-1β
DescriptionRecombinant, human interleukin-1β expressed in E. coli. Major form of interleukin-1 secreted by monocytes and macrophages. IL-1β is activated by proteolytic cleavage of the precursor molecule. Increases nitric oxide production in smooth muscle cells and suppresses apoptosis in rat ovarian follicles. Involved in inflammatory and immune responses and promotes wound healing. Induces nitric oxide synthase in insulin-producing cells. 1 µg of IL-1β corresponds to 1.9 x 105 WHO units.
FormLyophilized
FormulationLyophilized from sterile-filtered PBS, 50 µg BSA/µg IL-1β.
CAS number97599-21-8
RTECSNM9740000
Purity≥97% by SDS-PAGE
ContaminantsEndotoxin: ≤1.0 EU/µg IL-1β
Biological activityED₅₀ = <12 pg/ml as measured in a cell proliferation assay using the murine T-cell line, D10.G4.1 (Symons, J.A. et al. 1987)
SolubilityReconstitute to a concentration of ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
Toxicity Standard Handling
Merck USA index14, 4993
ReferencesChun, S.Y., et al. 1995. Endocrinology 136, 3120.
Kunz, D., et al. 1994. Biochem. J. 304, 337.
Eizirik, D.L., et al. 1993. FEBS Lett. 317, 62.
Dinarello, C>A> 1991. Blood 77, 1627.
Casagli, M.C., et al. 1989. Biochem. Biophys. Res. Commun. 162, 357.
Symons, J.A., et al. 1987. In Lymphokines and interferons, A Practical Approach (Clemens, M.J., et al. eds.) IRL Press, Oxford p. 272.