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420119 JNK Inhibitor II - CAS 129-56-6 - Calbiochem

420119
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

CAS #Empirical Formula
129-56-6C₁₄H₈N₂O

Products

Numero di catalogoConfezionamento Qtà/conf
420119-25MG Fiala di plastica 25 mg
420119-50MG Fiala di plastica 50 mg
420119-5MG Fiala di plastica 5 mg
Description
OverviewA potent, cell-permeable, selective, and reversible inhibitor of c-Jun N-terminal kinase (JNK) (IC50 = 40 nM for JNK-1 and JNK-2 and 90 nM for JNK-3). The inhibition is competitive with respect to ATP. Exhibits over 300-fold greater selectivity for JNK as compared to ERK1 and p38-2 MAP kinases. Inhibits the phosphorylation of c-Jun and blocks cellular expression of IL-2, IFN-γ, TNF-α, and COX-2. Blocks IL-1-induced accumulation of phospho-Jun and induction of c-Jun transcription. A 50 mM (5 mg/454 µl) solution of JNK Inhibitor II (Cat. No. 420128) in DMSO is also available.
Catalogue Number420119
Brand Family Calbiochem®
SynonymsSAPK Inhibitor II, Anthra[1,9-cd]pyrazol-6(2H)-one, 1,9-pyrazoloanthrone, SP600125
References
ReferencesShin, M., and Boyd, Y.D. 2002. Biochim. Biophys. Acta 1589, 311.
Bennett, B.L., et al. 2001. Proc. Natl. Acad. Sci. USA 98, 13681.
Han, Z., et al. 2001. J. Clin. Invest. 108, 73.
Product Information
CAS number129-56-6
ATP CompetitiveY
FormYellowish orange solid
Hill FormulaC₁₄H₈N₂O
Chemical formulaC₁₄H₈N₂O
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ200
Applications
ApplicationJNK Inhibitor II, CAS 129-56-6, is a cell-permeable, potent, selective, ATP-competitive, and reversible inhibitor of c-Jun N-terminal kinase (IC50 = 40 nM for JNK-1 & 2 & 90 nM for JNK-3).
Biological Information
Primary TargetJNK 1, JNK 2, JNK 3
Primary Target IC<sub>50</sub>40 nM for JNK-1 and JNK-2 and 90 nM for JNK-3
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSCB4585000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 2 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

JNK Inhibitor II - CAS 129-56-6 - Calbiochem MSDS

Titolo

Scheda di sicurezza (MSDS) 

JNK Inhibitor II - CAS 129-56-6 - Calbiochem Certificati d'Analisi

TitoloNumero di lotto
420119

Riferimenti bibliografici

Panoramica delle referenze
Shin, M., and Boyd, Y.D. 2002. Biochim. Biophys. Acta 1589, 311.
Bennett, B.L., et al. 2001. Proc. Natl. Acad. Sci. USA 98, 13681.
Han, Z., et al. 2001. J. Clin. Invest. 108, 73.

Citazioni

Titolo
  • Alexandra Thiel, et al. (2006) Expression of Cyclooxygenase-2 Is Regulated by Glycogen Synthase Kinase-3beta in Gastric Cancer Cells. Journal of Biological Chemistry 281, 4564-4569.
  • Yuseok Moon, Wayne C. Glasgow and Thomas E. Eling. (2005) Curcumin suppresses interleukin 1β-mediated microsomal prostaglandin E synthase 1 (mPGES-1) by altering early growth response gene EGR-1 and other signaling pathways. Journal of Pharmacology and Experimental Therapeutics in press,.
  • Naoto Inukai, et al. (2004) A novel hydrogen peroxide-induced phosphorylation and ubiquitination pathway leading to RNA polymerase II proteolysis. Journal of Biological Chemistry 279, 8190-8195.
  • Scheda tecnica

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision08-June-2009 RFH
    SynonymsSAPK Inhibitor II, Anthra[1,9-cd]pyrazol-6(2H)-one, 1,9-pyrazoloanthrone, SP600125
    DescriptionA potent, cell-permeable, selective, and reversible inhibitor of c-Jun N-terminal kinase (JNK) (IC50 = 40 nM for JNK-1 and JNK-2 and 90 nM for JNK-3). The inhibition is competitive with respect to ATP. Exhibits over 300-fold greater selectivity for JNK as compared to ERK1 and p38-2 MAP kinases. Inhibits the phosphorylation of c-Jun and blocks the expression of IL-2, IFN-γ, TNF-α, and COX-2 in cells. Blocks IL-1-induced accumulation of phospho-Jun and induction of c-Jun transcription.
    FormYellowish orange solid
    Intert gas (Yes/No) Packaged under inert gas
    CAS number129-56-6
    RTECSCB4585000
    Chemical formulaC₁₄H₈N₂O
    Structure formulaStructure formula
    Purity≥98% by HPLC
    SolubilityDMSO (15 mg/ml). For every 10 µM concentration of JNK Inhibitor II, include 0.1% DMSO in the culture medium. Pre-warming of culture medium and addition of BSA to the medium may enhance its solubility.
    Storage -20°C
    Protect from light
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 2 months at -20°C.
    Toxicity Standard Handling
    ReferencesShin, M., and Boyd, Y.D. 2002. Biochim. Biophys. Acta 1589, 311.
    Bennett, B.L., et al. 2001. Proc. Natl. Acad. Sci. USA 98, 13681.
    Han, Z., et al. 2001. J. Clin. Invest. 108, 73.
    Citation
  • Alexandra Thiel, et al. (2006) Expression of Cyclooxygenase-2 Is Regulated by Glycogen Synthase Kinase-3beta in Gastric Cancer Cells. Journal of Biological Chemistry 281, 4564-4569.
  • Yuseok Moon, Wayne C. Glasgow and Thomas E. Eling. (2005) Curcumin suppresses interleukin 1β-mediated microsomal prostaglandin E synthase 1 (mPGES-1) by altering early growth response gene EGR-1 and other signaling pathways. Journal of Pharmacology and Experimental Therapeutics in press,.
  • Naoto Inukai, et al. (2004) A novel hydrogen peroxide-induced phosphorylation and ubiquitination pathway leading to RNA polymerase II proteolysis. Journal of Biological Chemistry 279, 8190-8195.