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454854 MAP Kinase 2, His•Tag®, Human, Recombinant, E. coli

454854
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Products

Numero di catalogoConfezionamento Qtà/conf
454854-5UG Fiala di plastica 5 μg
Description
OverviewRecombinant, human MAP Kinase 2 fused to a His•Tag® sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified MAP Kinase in vitro with MEK1. Suitable for labeling MAP Kinase substrates.
Catalogue Number454854
Brand Family Calbiochem®
SynonymsERK2, MAPK2, p42MAPK
References
ReferencesLew, J. 2003. Biochemistry 42, 849.
Johnson, G.L., and Lapadat, R. 2002. Science 298, 1911.
Koistinaho, M., and Koistinaho, J. 2002. Glia 40, 175.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein (MBP) per min at 30°C.
FormLiquid
FormulationIn 150 mM NaCl, 50 mM Tris, 1 mM DTT, 50% glycerol, pH 8.5.
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by SDS-PAGE
Specific Activity≥5500 units/mg protein
Physicochemical Information
ContaminantsProteases: none detected
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

MAP Kinase 2, His•Tag®, Human, Recombinant, E. coli MSDS

Titolo

Scheda di sicurezza (MSDS) 

MAP Kinase 2, His•Tag®, Human, Recombinant, E. coli Certificati d'Analisi

TitoloNumero di lotto
454854

Riferimenti bibliografici

Panoramica delle referenze
Lew, J. 2003. Biochemistry 42, 849.
Johnson, G.L., and Lapadat, R. 2002. Science 298, 1911.
Koistinaho, M., and Koistinaho, J. 2002. Glia 40, 175.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-August-2008 RFH
SynonymsERK2, MAPK2, p42MAPK
DescriptionRecombinant, human MAP Kinase 2 fused to a His•Tag® sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified MAP kinase in vitro with MEK1. Suitable for labeling MAP Kinase substrates.
FormLiquid
FormulationIn 150 mM NaCl, 50 mM Tris, 1 mM DTT, 50% glycerol, pH 8.5.
Purity≥95% by SDS-PAGE
ContaminantsProteases: none detected
Specific activity≥5500 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein (MBP) per min at 30°C.
Storage ≤ -70°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesLew, J. 2003. Biochemistry 42, 849.
Johnson, G.L., and Lapadat, R. 2002. Science 298, 1911.
Koistinaho, M., and Koistinaho, J. 2002. Glia 40, 175.