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475858 Met30 Antagonist, SMER3 - Calbiochem

475858
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Panoramica

Replacement Information

Tabella delle specifiche principali

Empirical Formula
C₁₁H₄N₄O₂

Products

Numero di catalogoConfezionamento Qtà/conf
475858-25MG Bottiglia di vetro 25 mg
Description
OverviewA cell-permeable indenofurazinopyrazinone compound that is shown to directly target the F-box protein Met30 subunit of the Skp1-cullin-F-box (SCF) E3 ubiquitin ligase complex, disrupting Met30-Skp1 interaction and selectively inhibiting SCFMet30, but not SCFCdc4, E3 ubiquitin ligase activity. SMER3 is reported to act in a synthetic lethal fashion with rapamycin (Cat. Nos. 553210, 553211, & 553212) in both human lung cancer A549 and in yeast cultures. SMER3 treatment (10 to 30 µM) results in cell cycle arrest and morphological phenotype resembling that seen with temperature-sensitive Met30, but not Skp1 or Cdc4, mutant under non-permissive temperature in yeast cultures.
Catalogue Number475858
Brand Family Calbiochem®
SynonymsSmall-Molecule Enhancer of Rapamycin-3, 9H-Indeno[1,2-b][1,2,5]oxadiazolo[3,4-e]pyrazin-9-one, Indeno[1,2-b]furazano[3,4-E]pyrazin-9-one, SCFMet30 Inhibitor
References
ReferencesAghajan, M., et al. 2010. Nature Biotechnol. 28, 738.
Product Information
FormYellow solid
Hill FormulaC₁₁H₄N₄O₂
Chemical formulaC₁₁H₄N₄O₂
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity≥97% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Regulatory Review
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

Met30 Antagonist, SMER3 - Calbiochem MSDS

Titolo

Scheda di sicurezza (MSDS) 

Met30 Antagonist, SMER3 - Calbiochem Certificati d'Analisi

TitoloNumero di lotto
475858

Riferimenti bibliografici

Panoramica delle referenze
Aghajan, M., et al. 2010. Nature Biotechnol. 28, 738.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision24-October-2011 RFH
SynonymsSmall-Molecule Enhancer of Rapamycin-3, 9H-Indeno[1,2-b][1,2,5]oxadiazolo[3,4-e]pyrazin-9-one, Indeno[1,2-b]furazano[3,4-E]pyrazin-9-one, SCFMet30 Inhibitor
DescriptionA cell-permeable indenofurazinopyrazinone compound that is shown to directly target the F-box protein Met30 subunit of the Skp1-cullin-F-box (SCF) E3 ubiquitin ligase complex, disrupting Met30-Skp1 interaction and selectively inhibiting SCFMet30, but not SCFCdc4, E3 ubiquitin ligase activity. SMER3 is reported to act in a synthetic lethal fashion with rapamycin (Cat. Nos. 553210, 553211, & 553212) in both human lung cancer A549 and in yeast cultures. SMER3 treatment (10 to 30 µM) results in cell cycle arrest and morphological phenotype resembling that seen with temperature-sensitive Met30, but not Skp1 or Cdc4, mutant under non-permissive temperature in yeast cultures.
FormYellow solid
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₁₁H₄N₄O₂
Structure formulaStructure formula
Purity≥97% by HPLC
SolubilityDMSO (10 mg/ml). It is recommended to prepare a stock solution for 3 month use each time and store the unreconstituted material in solid form, protected from moisture and preferably under inert gas, for best long-term stability during storage.
Storage +2°C to +8°C
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Regulatory Review
ReferencesAghajan, M., et al. 2010. Nature Biotechnol. 28, 738.