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411455 Mouse Anti-Human IgG₁, Fc Fragment Specific (HP6070)

411455
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Products

Numero di catalogoConfezionamento Qtà/conf
411455-400UG Fiala di plastica 400 μg
Description
Catalogue Number411455
Brand Family Calbiochem®
References
ReferencesHamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
Jefferies, R., et al. 1986. Immunol. Letters 10, 223.
Product Information
FormLiquid
FormulationAschites diluted in PBS, pH 7.4.
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesELISA Hamilton, R.G. 1987. Clin. Chem. 33, 1707.
Application NotesELISA (see comments and application references)
Application CommentsCan be used as a capture antibody for ELISA at 10 µg/ml in PBS without carrier protein. Antibody should be titrated for optimal results in individual systems.

Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: 100%
Human IgG2: 0.1%
Human IgG3: 0.1%
Human IgG4: ≤0.02%
Human IgA: ≤0.1%
Human IgE: ≤0.1%
Human IgM: ≤0.1%

Cross-reactivity by ELISA against chimeric antibodies:
Human IgG1: 100%
Human IgG2: <0.01%
Human IgG3: <0.01%
Human IgG4: <0.01%
Human IgA: <0.01%
Human IgE: <0.001%
Human IgM: <0.01%
Biological Information
CloneHP6070
HostMouse
IsotypeIgG₁
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Mouse Anti-Human IgG₁, Fc Fragment Specific (HP6070) Certificati d'Analisi

TitoloNumero di lotto
411455

Riferimenti bibliografici

Panoramica delle referenze
Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
Jefferies, R., et al. 1986. Immunol. Letters 10, 223.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-August-2007 JSW
ApplicationELISA (see comments and application references)
DescriptionMouse monoclonal antibody supplied as diluted ascites. Recognizes human IgG1 Fc fragment.
BackgroundThe HP series of murine human Ig-specific monoclonal antibodies was produced from documented hybridoma cell lines that were developed at the Centers for Disease Control in Atlanta, Georgia. Laboratory analysis of each monoclonal antibody lot is performed in two stages during the production of ascites. First, the cell culture medium is analyzed for the presence and relative amount of human Ig-specific antibody by ELISA before injection of cells into mice. Second, collected ascites are analyzed for potency and antibody specificity using ELISA and isoelectric focusing (IEF), often in combination with an affinity immunoblot. For more information, the Calbiochem® technical monograph, The Human IgG Subclasses, is available free of charge.
HostMouse
CloneHP6070
IsotypeIgG₁
FormLiquid
FormulationAschites diluted in PBS, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsCan be used as a capture antibody for ELISA at 10 µg/ml in PBS without carrier protein. Antibody should be titrated for optimal results in individual systems.

Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: 100%
Human IgG2: 0.1%
Human IgG3: 0.1%
Human IgG4: ≤0.02%
Human IgA: ≤0.1%
Human IgE: ≤0.1%
Human IgM: ≤0.1%

Cross-reactivity by ELISA against chimeric antibodies:
Human IgG1: 100%
Human IgG2: <0.01%
Human IgG3: <0.01%
Human IgG4: <0.01%
Human IgA: <0.01%
Human IgE: <0.001%
Human IgM: <0.01%
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesHamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
Jefferies, R., et al. 1986. Immunol. Letters 10, 223.
Application referencesELISA Hamilton, R.G. 1987. Clin. Chem. 33, 1707.