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PS1008 PhosphoDetect™ Anti-FAK (pTyr⁸⁶¹) Rabbit pAb

PS1008
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Species ReactivityHostAntibody Type
Ch, H, M Rb Polyclonal Antibody

Products

Numero di catalogoConfezionamento Qtà/conf
PS1008-100UL Fiala di plastica 100 ul
Description
OverviewRecognizes the ~125 kDa FAK protein phosphorylated at Tyr861 in primary chick embryo fibroblasts transfected with human FAK.
Catalogue NumberPS1008
Brand Family Calbiochem®
SynonymsAnti-FADK1, Phospho-Specific (Tyr⁸⁶¹), Anti-pp125FAK, Phospho-Specific (Tyr⁸⁶¹), Anti-Protein Tyrosine Kinase 2, Phospho-Specific (Tyr⁸⁶¹), Anti-PTK2, Phospho-Specific (Tyr⁸⁶¹), FAK 1
Application Data
Detection of FAK, phosphorylated at Tyr861, by immunoblotting. Samples: Whole cell lysates from chicken embryo fibroblasts (CEF) expressing FAK and plated on fibronectin. Primary antibody: PhosphoDetect™ Anti-FAK (pTyr861) Rabbit pAb (Cat. No. PS1008) (1:1000) pre-incubated with phosphopeptide immunogen (lane 1), a generic phosphotyrosine-containing peptide (lane 2), non-phosphorylated peptide corresponding to the phosphopeptide immunogen (lane 3), or without peptide (lane 4). Detection: chemiluminescence.
References
ReferencesEliceiri, B.P., et al. 2002. J. Cell. Biol. 157, 149.
Hauck, C.R., et al. 2002. J. Biol. Chem. 277, 12487.
Lacalle, R.A., et al. 2002. J. Cell. Biol. 157, 277.
Lim, Y., et al. 2002. J. Biol. Chem. 277, 12735.
Melendez, J., et al. 2002. J. Biol. Chem. 227, 45203.
Rigacci, S., et al. 2002. J. Biol. Chem. 277, 41631.
Datta, A., et al. 2001. Mol. Cell. Biol. 21, 7295.
Nakamura, K., et al. 2001. Oncogene 20, 2626.
Rocic, P., et al. 2001. J. Biol. Chem. 276, 21902.
Slack, J.K., et al. 2001. Oncogene 20, 1152.
Sieg, D.J., et al. 2000. Nature Cell. Biol. 2, 249.
Vial, D., et al. 2000. J. Biol. Chem. 275, 28269.
Wennerberg, K., et al 2000. Mol. Cell. Biol. 20, 5758.
Product Information
FormLiquid
FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50 % glycerol, 1 mg/ml BSA, pH 7.3 (±0.1).
Positive controlChicken embryo fibroblasts (CEF) expressing FAK, plated on fibronectin
Preservative≤0.1 sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunohistochemistry
Application NotesImmunoblotting (1:1000)
Immunohistochemistry (see comments)
Application CommentsThe immunogen sequence is 100% conserved in rat and Xenopus FAK but cross-reactivity has not been tested. This antibody is also reported to work for immunohistochemistry. Antibody should be titrated for optimal use in individual systems.
Biological Information
Immunogena synthetic phosphopeptide surrounding the Tyr⁸⁶¹ phosphorylation site of human FAK
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Chicken
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Irritant
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

PhosphoDetect™ Anti-FAK (pTyr⁸⁶¹) Rabbit pAb MSDS

Titolo

Scheda di sicurezza (MSDS) 

PhosphoDetect™ Anti-FAK (pTyr⁸⁶¹) Rabbit pAb Certificati d'Analisi

TitoloNumero di lotto
PS1008

Riferimenti bibliografici

Panoramica delle referenze
Eliceiri, B.P., et al. 2002. J. Cell. Biol. 157, 149.
Hauck, C.R., et al. 2002. J. Biol. Chem. 277, 12487.
Lacalle, R.A., et al. 2002. J. Cell. Biol. 157, 277.
Lim, Y., et al. 2002. J. Biol. Chem. 277, 12735.
Melendez, J., et al. 2002. J. Biol. Chem. 227, 45203.
Rigacci, S., et al. 2002. J. Biol. Chem. 277, 41631.
Datta, A., et al. 2001. Mol. Cell. Biol. 21, 7295.
Nakamura, K., et al. 2001. Oncogene 20, 2626.
Rocic, P., et al. 2001. J. Biol. Chem. 276, 21902.
Slack, J.K., et al. 2001. Oncogene 20, 1152.
Sieg, D.J., et al. 2000. Nature Cell. Biol. 2, 249.
Vial, D., et al. 2000. J. Biol. Chem. 275, 28269.
Wennerberg, K., et al 2000. Mol. Cell. Biol. 20, 5758.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-October-2007 RFH
SynonymsAnti-FADK1, Phospho-Specific (Tyr⁸⁶¹), Anti-pp125FAK, Phospho-Specific (Tyr⁸⁶¹), Anti-Protein Tyrosine Kinase 2, Phospho-Specific (Tyr⁸⁶¹), Anti-PTK2, Phospho-Specific (Tyr⁸⁶¹), FAK 1
ApplicationImmunoblotting (1:1000)
Immunohistochemistry (see comments)
Application Data
Detection of FAK, phosphorylated at Tyr861, by immunoblotting. Samples: Whole cell lysates from chicken embryo fibroblasts (CEF) expressing FAK and plated on fibronectin. Primary antibody: PhosphoDetect™ Anti-FAK (pTyr861) Rabbit pAb (Cat. No. PS1008) (1:1000) pre-incubated with phosphopeptide immunogen (lane 1), a generic phosphotyrosine-containing peptide (lane 2), non-phosphorylated peptide corresponding to the phosphopeptide immunogen (lane 3), or without peptide (lane 4). Detection: chemiluminescence.
DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site and a generic phosphotyrosine-containing peptide, followed by immunoaffinity chromatography. Recognizes the ~125 kDa FAK protein phosphorylated at Tyr861.
BackgroundFocal adhesion kinase (FAK) is a non-receptor protein tyrosine kinase that plays a key role in maintaining focal adhesion function and cell survival. It is phosphorylated following growth factor or integrin-mediated cell adhesion stimulation. FAK is localized to focal adhesions through its C-terminal focal adhesion targeting (FAT) domain that acts by binding to paxillin and talin that are associated with the cytoplasmic tails of integrins. Phosphorylation of FAK at Tyr861 by Src promotes this interaction between FAK and integrins.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic phosphopeptide surrounding the Tyr⁸⁶¹ phosphorylation site of human FAK
IsotypeIgG
Specieschicken, human, mouse
Positive controlChicken embryo fibroblasts (CEF) expressing FAK, plated on fibronectin
FormLiquid
FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50 % glycerol, 1 mg/ml BSA, pH 7.3 (±0.1).
Preservative≤0.1 sodium azide
CommentsThe immunogen sequence is 100% conserved in rat and Xenopus FAK but cross-reactivity has not been tested. This antibody is also reported to work for immunohistochemistry. Antibody should be titrated for optimal use in individual systems.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Irritant
ReferencesEliceiri, B.P., et al. 2002. J. Cell. Biol. 157, 149.
Hauck, C.R., et al. 2002. J. Biol. Chem. 277, 12487.
Lacalle, R.A., et al. 2002. J. Cell. Biol. 157, 277.
Lim, Y., et al. 2002. J. Biol. Chem. 277, 12735.
Melendez, J., et al. 2002. J. Biol. Chem. 227, 45203.
Rigacci, S., et al. 2002. J. Biol. Chem. 277, 41631.
Datta, A., et al. 2001. Mol. Cell. Biol. 21, 7295.
Nakamura, K., et al. 2001. Oncogene 20, 2626.
Rocic, P., et al. 2001. J. Biol. Chem. 276, 21902.
Slack, J.K., et al. 2001. Oncogene 20, 1152.
Sieg, D.J., et al. 2000. Nature Cell. Biol. 2, 249.
Vial, D., et al. 2000. J. Biol. Chem. 275, 28269.
Wennerberg, K., et al 2000. Mol. Cell. Biol. 20, 5758.