Attenzione: Ci siamo trasferiti. Non è più possibile acquistare i prodotti Merck Millipore nel sito MerckMillipore.comPer saperne di più

442706 PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr²⁰²/Tyr²⁰⁴) Mouse mAb (12D4)

442706
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Species ReactivityHostAntibody Type
H, M, R M Monoclonal Antibody

Products

Numero di catalogoConfezionamento Qtà/conf
442706-1SET Fiala di plastica 1 set
Description
OverviewRecognizes the ~42-44 kDa ERK1 and ERK2 proteins phosphorylated at Thr202 and Tyr204 in vanadate-treated A431 cells. Supplied with a control lysate.
Catalogue Number442706
Brand Family Calbiochem®
SynonymsAnti-MAPK1/2, Activated
References
Product Information
FormLyophilized
Formulation100 µg antibody lyophilized from 2X PBS, sucrose, polyethylene glycol (PEG) and 150 µl lyophilized control lysate from 1 x 10⁶ vanadate-treated A431 cells.
Positive controlvanadate-treated A431 cells (supplied)
Preservative≤0.1% sodium azide (antibody only)
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesELISA (0.05 µg/ml)
Immunoblotting (1 µg/ml, colorimetric or 0.1 µg/ml, chemiluminescence)
Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
Application CommentsAntibody requires phosphorylation at both the Thr and Tyr site for recognition. Does not recognize non-phosphorylated MAP kinase 1/2. Does not cross-react with SAPK 1 or SAPK2 proteins. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Thr204/204 phosphorylation stie of mouse ERK1 and ERK2
ImmunogenHuman
Clone12D4
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsReconstitute antibody with 1 ml dH₂O. Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Reconstitute the positive control cell lysate with 200 µl dH₂O. Following complete solubilization add 200 µl 2X SDS-PAGE sample buffer and incubate at 90°C for 5 min. Aliquot and freeze (-20°C). Avoid freeze/thaw cycles of reconstituted antibody and control lysate.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr²⁰²/Tyr²⁰⁴) Mouse mAb (12D4) Certificati d'Analisi

TitoloNumero di lotto
442706
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-August-2007 RFH
SynonymsAnti-MAPK1/2, Activated
ApplicationELISA (0.05 µg/ml)
Immunoblotting (1 µg/ml, colorimetric or 0.1 µg/ml, chemiluminescence)
Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
DescriptionMouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the ~42-44 kDa ERK1 and ERK2 protein phosphorylated on Thr202/Tyr204. Supplied with control lysate consisting of 1 x 106 vanadate-treated A431 cells.
HostMouse
Immunogen speciesHuman
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Thr204/204 phosphorylation stie of mouse ERK1 and ERK2
Clone12D4
IsotypeIgG₁
Specieshuman, mouse, rat
Positive controlvanadate-treated A431 cells (supplied)
FormLyophilized
Formulation100 µg antibody lyophilized from 2X PBS, sucrose, polyethylene glycol (PEG) and 150 µl lyophilized control lysate from 1 x 10⁶ vanadate-treated A431 cells.
Preservative≤0.1% sodium azide (antibody only)
CommentsAntibody requires phosphorylation at both the Thr and Tyr site for recognition. Does not recognize non-phosphorylated MAP kinase 1/2. Does not cross-react with SAPK 1 or SAPK2 proteins. Variables associated with assay conditions will dictate the proper working dilution.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute antibody with 1 ml dH₂O. Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Reconstitute the positive control cell lysate with 200 µl dH₂O. Following complete solubilization add 200 µl 2X SDS-PAGE sample buffer and incubate at 90°C for 5 min. Aliquot and freeze (-20°C). Avoid freeze/thaw cycles of reconstituted antibody and control lysate.
Toxicity Standard Handling