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442685 PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr²⁰²/Tyr²⁰⁴) Rabbit pAb

442685
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Species ReactivityHostAntibody Type
H, R Rb Polyclonal Antibody

Products

Numero di catalogoConfezionamento Qtà/conf
442685-100UL Fiala di plastica 100 ul
Description
OverviewRecognizes the ~42-44 kDa ERK1 and ERK2 proteins phosphorylated at Thr202 and Tyr204.
Catalogue Number442685
Brand Family Calbiochem®
Application Data
Detection of human MAP Kinase ERK1/ERK2, phosphorylated on Thr202/Tyr204, by immunoblotting. Samples: Whole cell lysate from human T47D cells untreated (lane 1) or treated with lambda-phosphatase (lane 2). Primary antibody: PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr202/Tyr204) Rabbit pAb (Cat. No. 442685) (1:1000). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 10 mM HEPES, 100 μg/ml BSA, 50% glycerol, pH 7.5.
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Dot Blot
Immunoblotting (Western Blotting)
Application NotesDot Blot (1:1000)
Immunoblotting (1:1000)
Application CommentsDoes not react with the unphosphorylated proteins. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic phosphopeptide surrounding the Thr²⁰²/Tyr²⁰⁴ phosphorylation sites of human ERK1/ERK2
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr²⁰²/Tyr²⁰⁴) Rabbit pAb MSDS

Titolo

Scheda di sicurezza (MSDS) 

PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr²⁰²/Tyr²⁰⁴) Rabbit pAb Certificati d'Analisi

TitoloNumero di lotto
442685
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-August-2007 RFH
ApplicationDot Blot (1:1000)
Immunoblotting (1:1000)
Application Data
Detection of human MAP Kinase ERK1/ERK2, phosphorylated on Thr202/Tyr204, by immunoblotting. Samples: Whole cell lysate from human T47D cells untreated (lane 1) or treated with lambda-phosphatase (lane 2). Primary antibody: PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr202/Tyr204) Rabbit pAb (Cat. No. 442685) (1:1000). Detection: chemiluminescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~42-44 kDa ERK1 and ERK2 proteins phosphorylated at Thr202 and Tyr204.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic phosphopeptide surrounding the Thr²⁰²/Tyr²⁰⁴ phosphorylation sites of human ERK1/ERK2
IsotypeIgG
Specieshuman, rat
FormLiquid
FormulationIn 150 mM NaCl, 10 mM HEPES, 100 μg/ml BSA, 50% glycerol, pH 7.5.
PreservativeNone
CommentsDoes not react with the unphosphorylated proteins. Variables associated with assay conditions will dictate the proper working dilution.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling