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PS1013 PhosphoDetect™ Anti-VEGFR2 (pTyr1054/1059) Rabbit pAb

PS1013
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Species ReactivityHostAntibody Type
MRbPolyclonal Antibody

Products

Numero di catalogoConfezionamento Qtà/conf
PS1013-10T Fiala di plastica 10 t
Description
OverviewRecognizes the ~200 kDa VEGFR-2 protein phosphorylated at Tyr1054/1059 in porcine pancreatic cells transfected with a CSF-1/mouse VEGFR-2 chimeric receptor.
Catalogue NumberPS1013
Brand Family Calbiochem®
SynonymsAnti-VEGF Receptor 2, Phospho-specific (pTyr1054/1059), Anti-KDR/Flk-1, Phospho-Specific (Tyr1054/1059)
Application Data
Detection of mouse VEGFR2, phosphorylated at Tyr1054/1059, by immunoblotting. Samples: Whole cell lysate from porcine aortic endothelial (PAE) cells transfected with CSF-1/mouse VEGFR2 chimeric receptor left unstimulated (lanes 1,2) or stimulated with CSF-1 (lanes 3-8). Primary antibodies: PhosphoDetect™ Anti-VEGFR2 (pTyr1054/1059) Rabbit pAb (Cat. No. PS1013) (0.5 µg/ml) pre-incubated without peptide (lanes 2,4,5), with non-phosphopeptide corresponding to the immunogen (lane 6), a generic phosphotyrosine-containing peptide (lane 7), or the phosphopeptide immunogen (lane 8) or pan anti-VEGFR2 antibody (lanes 1,3). Detection: chemiluminescence.
References
ReferencesMeyer, R.D., et al. 2002. J. Biol. Chem. 277, 27081.
Zeng, H., et al. 2001. J. Biol. Chem. 276, 32714.
Sato, Y., et al. 2000. Ann. N.Y. Acad. Sci. 902, 201.
Rahimi, N., et al. 1999. Mol. Biol. Cell 10, 3401.
Product Information
FormLiquid
FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3 (± 0.1).
Positive controlPorcine aortic endothelial (PAE) cells transfected with a CSF-1/mouse VEGFR2 chimeric receptor
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:1000)
Application CommentsThe immunogen sequence is 100% conserved in human and rat VEGFR2, but reactivity has not been tested. The corresponding phosphorylation site in VEGFR3 is 100% conserved, but cross-reactivity has not been tested. When used at a 1:1000 dilution there is sufficient antibody for 10 immunoblots at 10 ml/blot. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1054/1059 phosphorylation sites of mouse and human VEGFR-2
HostRabbit
IsotypeIgG
Species Reactivity
  • Mouse
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Irritant
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
PS1013-10T 04055977227086

Documentation

PhosphoDetect™ Anti-VEGFR2 (pTyr1054/1059) Rabbit pAb Certificati d'Analisi

TitoloNumero di lotto
PS1013

Riferimenti bibliografici

Panoramica delle referenze
Meyer, R.D., et al. 2002. J. Biol. Chem. 277, 27081.
Zeng, H., et al. 2001. J. Biol. Chem. 276, 32714.
Sato, Y., et al. 2000. Ann. N.Y. Acad. Sci. 902, 201.
Rahimi, N., et al. 1999. Mol. Biol. Cell 10, 3401.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision15-August-2007 RFH
SynonymsAnti-VEGF Receptor 2, Phospho-specific (pTyr1054/1059), Anti-KDR/Flk-1, Phospho-Specific (Tyr1054/1059)
ApplicationImmunoblotting (1:1000)
Application Data
Detection of mouse VEGFR2, phosphorylated at Tyr1054/1059, by immunoblotting. Samples: Whole cell lysate from porcine aortic endothelial (PAE) cells transfected with CSF-1/mouse VEGFR2 chimeric receptor left unstimulated (lanes 1,2) or stimulated with CSF-1 (lanes 3-8). Primary antibodies: PhosphoDetect™ Anti-VEGFR2 (pTyr1054/1059) Rabbit pAb (Cat. No. PS1013) (0.5 µg/ml) pre-incubated without peptide (lanes 2,4,5), with non-phosphopeptide corresponding to the immunogen (lane 6), a generic phosphotyrosine-containing peptide (lane 7), or the phosphopeptide immunogen (lane 8) or pan anti-VEGFR2 antibody (lanes 1,3). Detection: chemiluminescence.
DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~200 kDa VEGFR2 protein phosphorylated at Tyr1054/1059.
BackgroundVascular Endothelial Growth Factor Receptor 2 (VEGFR2) is receptor tyrosine kinase that serves as a key mediator in angiogenesis and plays an essential role in embryonic development, wound healing, cell proliferation, migration, and differentiation. It contains seven immunoglobulin-like sequences in the extracellular domain and exhibits sequence similarity to Flt-1 (FMS-like tyrosine kinase 1) and Flt-4 (FMS-like tyrosine kinase 4). Upon binding to its ligand, VEGF, VEGFR2 undergoes ligand-induced dimerization, which activates its intrinsic tyrosine kinase activity resulting in auto- and trans-phosphorylation on specific tyrosine residues in the cytoplasmic domain. These tyrosine residues, when phosphorylated, are involved as docking sites to recruit molecules containing Src homology 2 (SH2) or phosphotyrosine-binding domains and to convey signals to downstream effectors. Phosphorylation of VEGFR2 at Tyr1054 and Tyr1059 within the activation loop is required for required for activation of VEGFR2 and enhances its intrinsic tyrosine kinase activity. Phosphorylation at Tyr1059 is also responsible for VEGF-induced intracellular Ca2+ mobilization.
HostRabbit
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1054/1059 phosphorylation sites of mouse and human VEGFR-2
IsotypeIgG
Speciesmouse
Positive controlPorcine aortic endothelial (PAE) cells transfected with a CSF-1/mouse VEGFR2 chimeric receptor
FormLiquid
FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3 (± 0.1).
Preservative≤0.1% sodium azide
CommentsThe immunogen sequence is 100% conserved in human and rat VEGFR2, but reactivity has not been tested. The corresponding phosphorylation site in VEGFR3 is 100% conserved, but cross-reactivity has not been tested. When used at a 1:1000 dilution there is sufficient antibody for 10 immunoblots at 10 ml/blot. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Irritant
ReferencesMeyer, R.D., et al. 2002. J. Biol. Chem. 277, 27081.
Zeng, H., et al. 2001. J. Biol. Chem. 276, 32714.
Sato, Y., et al. 2000. Ann. N.Y. Acad. Sci. 902, 201.
Rahimi, N., et al. 1999. Mol. Biol. Cell 10, 3401.