Millipore Sigma Vibrant Logo
Attenzione: Ci siamo trasferiti. Non è più possibile acquistare i prodotti Merck Millipore nel sito MerckMillipore.comPer saperne di più

544049 Pyrintegrin - Calbiochem

544049
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Products

Numero di catalogoConfezionamento Qtà/conf
544049-5MG Bottiglia di vetro 5 mg
Description
OverviewA cell-permeable pyrimidine compound that promotes the adhesion of individually dissociated hESCs on matrigel- or laminin-, but not gelatin-coated surfaces and substantially reduces trypsinization-induced apoptosis (10% vs 81% TUNEL-positive cells from 2 µM Ptn or DMSO control matrigel cultures, respectively, after trypsinization), while maintaining cells’ self-renewal ability (colony formation rate = 25% or 1% 4 d after low density seeding of single-cells on matrigel surface with 2 µM Ptn or DMSO control, respectively) and the pluripotency of expanded cultures for more than 40 passages. Ptn treatment is shown to induce the activation of β1 integrin and multiple growth factor receptors, including FGFR1, IGFR1, EGFR1, and ErbB2, while blockage of β1 integrin, PI 3-K, or growth factor receptors abolishes the survival promoting effect of Ptn. Single-cell dissociation is demonstrated to hyperactivate Rho-ROCK signaling, while efficient hESC-ECM interaction suppresses Rho-Rock activation, resulting in the stabilization of newly synthesized cell surface E-cadherin, which in turn promotes cell-cell adhesion, an important determinant of hESC survival.
Catalogue Number544049
Brand Family Calbiochem®
SynonymsPtn, N-(Cyclopropylmethyl)-4-(4-(6-hydroxy-3,4-dihydroquinolin-1(2H)-yl)pyrimidin-2-ylamino)benzenesulfonamide, β1 Integrin Activator
References
ReferencesXu, Y., et al. 2010. Proc. Natl. Acad. Sci. USA 107, 8129.
Product Information
FormWhite solid
Hill FormulaC₂₃H₂₅N₅O₃S
Chemical formulaC₂₃H₂₅N₅O₃S
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

Pyrintegrin - Calbiochem MSDS

Titolo

Scheda di sicurezza (MSDS) 

Pyrintegrin - Calbiochem Certificati d'Analisi

TitoloNumero di lotto
544049

Riferimenti bibliografici

Panoramica delle referenze
Xu, Y., et al. 2010. Proc. Natl. Acad. Sci. USA 107, 8129.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision31-August-2011 RFH
SynonymsPtn, N-(Cyclopropylmethyl)-4-(4-(6-hydroxy-3,4-dihydroquinolin-1(2H)-yl)pyrimidin-2-ylamino)benzenesulfonamide, β1 Integrin Activator
DescriptionA cell-permeable pyrimidine compound that promotes the adhesion of individually dissociated hESCs on matrigel- or laminin-, but not gelatin-coated surfaces and substantially reduces trypsinization-induced apoptosis (10% vs 81% TUNEL-positive cells from 2 µM Ptn or DMSO control matrigel cultures, respectively, after trypsinization), while maintaining cells' self-renewal ability (colony formation rate = 25% or 1% 4 d after low density seeding of single-cells on matrigel surface with 2 µM Ptn or DMSO control, respectively) and the pluripotency of expanded cultures for more than 40 passages. Ptn treatment is shown to induce the activation of β1 integrin and multiple growth factor receptors, including FGFR1, IGFR1, EGFR1, and ErbB2, while blockage of β1 integrin, PI 3-K, or growth factor receptors abolishes the survival promoting effect of Ptn. Single-cell dissociation is demonstrated to hyperactivate Rho-ROCK signaling, while efficient hESC-ECM interaction suppresses Rho-Rock activation, resulting in the stabilization of newly synthesized cell surface E-cadherin, which in turn promotes cell-cell adhesion, an important determinant of hESC survival.
FormWhite solid
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₂₃H₂₅N₅O₃S
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityDMSO (100 mg/ml)
Storage +2°C to +8°C
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesXu, Y., et al. 2010. Proc. Natl. Acad. Sci. USA 107, 8129.