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69671 Thrombin, Restriction Grade

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Replacement Information


Numero di catalogoConfezionamento Qtà/conf
69671-3 Fiala di plastica 50 u
OverviewRestriction Grade Thrombin is a highly purified preparation qualified to specifically cleave target proteins produced with appropriate vectors. This preparation is functionally tested for activity with fusion proteins and is free of detectable contaminating proteases. Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. Thrombin is supplied as a solution in 50% glycerol and includes 10X Thrombin Cleavage Buffer and a Cleavage Control Protein.
Catalogue Number69671
Brand Family Novagen®
Product Information
CAS number9002-04-4
Unit of DefinitionOne unit is defined as the amount of enzyme needed to cleave 1 mg of fusion protein in 16 hours at 20°C in a 200 µl reaction containing 20 mM Tris-HCl pH 8.4, 150 mM NaCl, 2.5 mM CaCl₂, 50 µg fusion protein.
Quality LevelMQ200
Biological Information
SourcePrepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Multiple Toxicity Values, refer to MSDS
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Global Trade Item Number
Numero di catalogo GTIN
69671-3 04055977274189


Thrombin, Restriction Grade Certificati d'Analisi

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Bulk Product Guide


  • Damien Bessière, et al. (2008) Structure-function analysis of the THAP zinc finger of THAP1, a large C2CH DNA-binding module linked to Rb/E2F pathways. Journal of Biological Chemistry 283, 4352-4363.
  • Amanda J. Davis, et al. (2007) Arabidopsis phosphatidylinositol phosphate kinase 1 binds F-actin and recruits phosphatidylinositol 4-kinase beta1 to the actin cytoskeleton. Journal of Biological Chemistry 282, 14121-14131.
  • Hua A. J. Lu, et al. (2007) Heat shock protein 70 interacts with aquaporin-2 and regulates its trafficking. Journal of Biological Chemistry 282, 28721-28732.
  • Hideaki Unno, et al. (2007) Structural and mutational studies of anthocyanin malonyltransferases establish the features of BAHD enzyme catalysis. Journal of Biological Chemistry 282, 15812-15822.
  • Fanny Boissier, et al. (2006) Further insight into S-adenosylmethionine-dependent methyltransferases: structural characterization of Hma, an enzyme essential for the biosynthesis of oxygenated mycolic acids in Mycobacterium tuberculosis. Journal of Biological Chemistry 281, 4434-4445.
  • Alyssa Carré-Mlouka, et al. (2006) A new rubisco-like protein coexists with a photosynthetic rubisco in the planktonic cyanobacteria Microcystis. Journal of Biological Chemistry 281, 24462-24471.
  • Hong Ye, et al. (2006) CpSufE activates the cysteine desulfurase CpNifS for chloroplastic Fe-S cluster formation. Journal of Biological Chemistry 281, 8958-8969.
  • Agnes E. Hamburger, et al. (2005) Steric accessibility of the HIV-1 gp41 N-trimer region. Journal of Biological Chemistry 280, 12567-12572.
  • Kenneth L. Madsen, et al. (2005) Molecular determinants for the complex binding specificity of the PDZ domain in PICK 1. Journal of Biological Chemistry 280, 20539-20548.
  • Frederic H. Vaillancourt, Jun Yin and Christopher T. Walsh. (2005) SyrB2 in syringomycin E biosynthesis is a nonheme FeII α-ketoglutarate- and O2-dependent halogenase. Proceedings of the National Academy of Sciences (USA) 102, 10111-10116.
  • Yasuo Yamakoshi, et al. (2005) Porcine dentin sialoprotein is a proteoglycan with glycosaminoglycan chains containing chondroitin 6-sulfate. Journal of Biological Chemistry 280, 1552-1560.
  • Yang Chao and Dax Fu. (2004) Thermodynamic studies of the mechanism of metal binding to the Escherichia coli zinc transporter YiiP. Journal of Biological Chemistry 279, 17173-17180.
  • Jeffrey M. Vargason, et al. (2004) Size selective recognition of siRNA by an RNA silencing suppressor. Cell 115, 799-811.
  • Marilyn H. Perrin, et al. (2003) A soluble form of the first extracellular domain of mouse type 2 corticotropin-releasing factor receptor reveals differential ligand specificity. Journal of Biological Chemistry 278, 15595-15600.
  • Songmin Cai and John H. Exton. (2001) Determination of interaction sites of phospholipase D1 for RhoA. Biochemical Journal 355, 779-785.
  • U. Vinkemeir, et al. (1998) Structure of the amino-terminal protein interaction domain of STST-4. Science 279, 1048-1052.
  • Alexy Bochkarev, et al. (1997) Structure of the single-stranded-DNA-binding domain of replication protein A bound to DNA. 385, 176-181.
  • Susan V. Pena, et al. (1997) Processing, subcellular localization, and function of 519 (granulysin), a human late T cell activation molecule with homology to small, lytic, granule proteins. Journal of Immunology 2680-2688.
  • John A. Lupisella, et al. (1995) The ligand binding domain of the human retinoic acid receptor Is predominantly α-helical with a trp residue in the ligand binding site. Journal of Biological Chemistry 270, 24884-24890.
  • Harikrishna Nakshatri, Poornima Nakshatri and R. Alexander Currie. (1995) Interaction of Oct-1 with TFIIB. Journal of Biological Chemistry 270, 19613-19623.
  • Kelvin Nurse, et al. (1995) Purification, cloning, and properties of the tRNA ψ55 synthase from Escherichia coli. RNA 1,.
  • Protocolli per l'uso

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