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69671 Thrombin, Restriction Grade

69671
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Products

Numero di catalogoConfezionamento Qtà/conf
69671-3 Fiala di plastica 50 u
Description
OverviewRestriction Grade Thrombin is a highly purified preparation qualified to specifically cleave target proteins produced with appropriate vectors. This preparation is functionally tested for activity with fusion proteins and is free of detectable contaminating proteases. Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. Thrombin is supplied as a solution in 50% glycerol and includes 10X Thrombin Cleavage Buffer and a Cleavage Control Protein.
Catalogue Number69671
Brand Family Novagen®
References
Product Information
CAS number9002-04-4
Unit of DefinitionOne unit is defined as the amount of enzyme needed to cleave 1 mg of fusion protein in 16 hours at 20°C in a 200 µl reaction containing 20 mM Tris-HCl pH 8.4, 150 mM NaCl, 2.5 mM CaCl₂, 50 µg fusion protein.
Components
Quality LevelMQ400
Applications
Biological Information
SourcePrepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSXO8950000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Multiple Toxicity Values, refer to MSDS
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Thrombin, Restriction Grade Certificati d'Analisi

TitoloNumero di lotto
69671

Brochure

Titolo
Bulk Product Guide

Citazioni

Titolo
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  • Kelvin Nurse, et al. (1995) Purification, cloning, and properties of the tRNA ψ55 synthase from Escherichia coli. RNA 1,.
  • Protocolli per l'uso

    Titolo
    TB188 Thrombin Kits