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70072 pET-32 Xa/LIC Vector Kit - Novagen

70072
Purchase on Sigma-Aldrich

Panoramica

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Products

Numero di catalogoConfezionamento Qtà/conf
70072-3 Bottiglia di vetro 20 rxn
Description
OverviewThe pET-32 Xa/LIC vector is an Xa/LIC version of pET-32b(+). It is designed for cloning and high-level expression of target proteins fused with the 109aa Trx•Tag™ thioredoxin protein, and His•Tag® and S®Tag™ coding sequences that are cleavable with Factor Xa protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the Xa protease cleavage site (IleGluGlyArg↓), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal His•Tag coding sequence is compatible with purification, detection, and quantification...




Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Catalogue Number70072
Brand Family Novagen®
References
Product Information
Components
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
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Supplemental Information
Specifications

Documentation

pET-32 Xa/LIC Vector Kit - Novagen Certificati d'Analisi

TitoloNumero di lotto
70072

Citazioni

Titolo
  • Hongjun Jin, et al. (2007) The human CC chemokine MIP-1 dimer is not competent to bind to the CCR5 receptor. Journal of Biological Chemistry 282, 27976-27983.
  • Geoffrey A. Preidis, et al. (2007) Seropositive human subjects produce interferon gamma after stimulation with recombinant Cryptosporidium hominis gp15. American Journal of Tropical Medicine and Hygiene 77, 583-585.
  • Beatrice Alpha-Bazin, et al. (2005) Boundaries and physical characterization of a new domain shared between mammalian 53BP1 and yeast Rad9 checkpoint proteins. Protein Science 14, 1827-1839.
  • Rey-Ting Guo, et al. (2005) Crystal structures of undecaprenyl pyrophosphate synthase in complex with magnesium, isopentenyl pyrophosphate and farnesyl thiopyrophosphate: roles of the metal ion and conserved residues in catalysis. Journal of Biological Chemistry 280, 20762-20774.
  • Min-Feng Hsu, et al. (2005) Understanding the maturation process and inhibitor design of SARS-CoV 3CLpro from the crystal structure of C145A in a product-bound form. Journal of Biological Chemistry 280, 31257-31266.
  • Savvas Saouros, et al. (2005) A novel galectin-like domain from Toxoplasma gondii micronemal protein 1 assists the folding, assembly, and transport of a cell-adhesion complex. Journal of Biological Chemistry 280, 38583-38591.
  • Atsushi Kodan, Hiroyuki Kuroda and Fukumi Sakai. (2002) A stilbene synthase from Japanese red pine (Pinus densiflora): Implications for phytoalexin accumulation and down-regulation of flavonoid biosynthesis. Procedings of the National Academy of Science 99, 3335-3339.
  • Protocolli per l'uso

    Titolo
    TB055 pET System Manual
    TB163 Ek/LIC Cloning Kits
    TB184 Xa/LIC Cloning Kits

    Mappa di un vettore

    Titolo
    TB186VM pET-32 Xa/LIC Vector Map