529625
Polyphosphoinositide-Binding Peptide, PBP10
A Rhodamine-B-labeled decapeptide derived from the (PIP2)-binding region in segment-2 of gelsolin.
Synonym(s):
Polyphosphoinositide-Binding Peptide, PBP10, RhoB-GS 160-169, Rhodamine B-QRLFQVKGRR-OH, RhoB-GS160-169, Rhodamine B-QRLFQVKGRR-OH
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About This Item
Empirical Formula (Hill Notation):
C84H127N24O15
CAS Number:
Molecular Weight:
1713.06
MDL number:
NACRES:
NA.77
UNSPSC Code:
12352211
Assay:
≥95% (HPLC)
Form:
lyophilized solid
Storage condition:
OK to freeze, desiccated (hygroscopic), protect from light
Quality Segment
assay
≥95% (HPLC)
form
lyophilized solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated (hygroscopic), protect from light
color
pink-red
solubility
DMSO: 2 mg/mL
fluorescence
λex 565 nm, λem 590 nm
shipped in
ambient
storage temp.
−20°C
General description
A Rhodamine-B-labeled decapeptide derived from the (PIP2)-binding region in segment-2 of gelsolin. Acts as a cell-permeable, reversible, and non-toxic agent that transiently disrupts actin filaments, which are essential for cell morphology and cell motility (~1-10 µM). Specifically binds to polyphosphoinositide (PPI) with high affinity and activates platelets without significantly affecting intracellular Ca2+ influx. Undergoes a reduction in fluorescence intensity upon binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid; the fluorescence is not affected in the presence of phosphatidylserine and phosphatidylcholine. Useful fluorescent probe for the cellular localization and stabilization of PPIs. Excitation max.: ~565 nm; emission max.: ~590 nm.
Biochem/physiol Actions
Cell permeable: yes
Primary Target
Specifically binds to polyphosphoinositide (PPI)
Specifically binds to polyphosphoinositide (PPI)
Product does not compete with ATP.
Reversible: yes
Packaging
Packaged under inert gas
Physical form
Supplied as a trifluoroacetate salt.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Other Notes
Bucki, R., et al. 2001. Biochemistry40, 15752
Cunningham, C.C., et al. 2001. J. Biol. Chem.276, 43390.
Cunningham, C.C., et al. 2001. J. Biol. Chem.276, 43390.
Rho-B-Gln-Arg-Leu-Phe-Gln-Val-Lys-Gly-Arg-Arg-OH
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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