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ABE386

Anti-Flightless-1 homolog Antibody

serum, from rabbit

Synonym(s):

Protein flightless-1 homolog, Fli1, Anti-Flightless-1 homolog Antibody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IP, WB
Citations:
-
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biological source

rabbit

Quality Segment

100

conjugate

unconjugated

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, rat, human

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

NCBI accession no.

NP_001243193

UniProt accession no.

Q13045

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... FLII(2314)

General description

Mammalian homolog of drosophila flightless-1 is encoded by the FLII gene in human (also known as FLI, Fli1, or FLIL). The human flightless-1 homolog (Flii) contains sixteen 23-amino acid leucine-rich repeats (LRR) in its N-terminal half and a gelsolin-like domain (GLD) in its C-terminal half, composed of six segmental repeats typical of many gelsolin family members. Flii is reported to interact with actin and "FLI LRR associated protein" (FLAP) via its GLD and LRR domain, respectively (PMID 9525888). Flii is also shown to interact with the glucose-responsive transcription factor carbohydrate responsive element binding protein (ChREBP) via both the LRR and GLD domains and functions as a negative regulator of ChREBP in cancer cells (PMID 24055811). Increased Flii level is observed in the blistered skin of patients with epidermolysis bullosa acquisita (EBA), and Flii overexpression in transgenic mice results in delayed epidermal barrier formation in embryos and decreased expression of tight junction (TJ) proteins, as well as impaired barrier function among adult transgenic mice (PMID 24375017).
~144 kDa observed

Immunogen

KLH-conjugated linear peptide corresponding to human Flightless-1 homolog.

Application

Detect Fli1 using this rabbit polyclonal antibody, Anti-Flightless-1 homolog Antibody validated for use in western blotting & IP.
Immunoprecipitation Analysis: A representative lot of this antibody detected Flightless-1 homolog in IP (Prof. D. Pallas, Emory University).

Analysis Note

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Flightless-1 homolog in 10 µg of A431 cell lysate.

Other Notes

Replaces: MABF195; MABE530

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Storage Class

10 - Combustible liquids

wgk

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ABE386:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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