ProtectRNA^™ RNase Inhibitor 500× Concentrate

Not recommended in systems where other enzymatic activity is required.

ProtectRNA^™ ribonuclease(RNase) Inhibitor inhibits most nucleic acid binding enzymes such as RNase and DNase, reverse transcriptase, some RNA and DNA polymerases, protein synthesis enzymes, and several restriction endonucleases. In contrast, ProtectRNA does not interfere with DNA and RNA hybridizations. The action ofProtectRNA as a nuclease inhibitor can be used as an advantage for in situ hybridization. ProtectRNA is useful during all steps of in situ hybridization for tissue RNA protection from RNase degradation. It is recommended to add ProtectRNA to all aqueous solutions used, from as early as the deparaffinization step until the hybridization is completed.

ProtectRNA^™ RNase Inhibitor 500× Concentrate has been used in:

• RNA extraction
• tissue sectioning and staining for laser capture microscopy
• the isolation of blood vessels from hippocampi

A potent inhibitor of most nucleic acid binding enzymes, and thus useful as an RNase inhibitor. Especially useful when performing in situ hybridization. If it is added to all aqueous solutions used, it eliminates the need for special glassware washing and after-wash treatments. The 500x concentrate is economical; 2 ml treats 1,000 ml of solution. Not recommended in systems where other enzymatic activity is required.

ProtectRNA^™ RNase Inhibitor 500× Concentrate is an RNase inhibitor.

2mL treats 1,000ml of solution.

This product is for R&D use only, not for drug, household, or other uses.

ProtectRNA is a trademark of Sigma-Aldrich Co. LLC