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SCM104 3dGRO™ R-Spondin-1 Conditioned Media Supplement

Alternative stem cell media supplement to purified recombinant R-Spondin-1 protein for organoid cultures.

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SCM104
50 mL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCM104
      Description3dGRO™ R-Spondin-1 Conditioned Media Supplement
      Alternate Names
      • RSPO1 Conditioned Media
      • RSPO1 CM
      Background Information3D organoid culture systems are increasingly employed as powerful tools for the study of human diseases. R-Spondin proteins are a family of cysteine-rich, thrombospondin type I repeat containing proteins that activate both the WNT/β-catenin and WNT/PCP pathways. R-spondin-1 (RSPO1) is one of the most extensively used niche factors for culturing 3D organoids and has been used to establish organoids from the stomach, small intestine, colon, pancreas and liver from both mouse and human sources1,2,3 along with intestinal organoids from other large and small animals4. However, due to the high concentration requirement in organoid media the use of the recombinant R-spondin-1 becomes prohibitively expensive. The 3dGRO™ R-Spondin-1 Conditioned Media Supplement is a potent and inexpensive alternative to purified recombinant RSPO1 protein for organoid cultures and is optimized for the highest level of RSPO-1 expression to ensure consistency and performance.

      Note: Product is supplied at a 2X concentration (see datasheet for a complete organoid media recipe).

      Source
      The 3dGRO™ R-Spondin-1 Conditioned Media Supplement is generated from a 293T cell transfectant stably secreting mouse R-spondin-1 tagged with an influenza hemagglutinin (HA) epitope at the N-terminus and a murine IgG2a Fc fragment at the C-terminus. The conditioned medium does not contain serum.
      References
      Product Information
      Applications
      ApplicationAlternative stem cell media supplement to purified recombinant R-Spondin-1 protein for organoid cultures.
      Key Applications
      • Cell Culture
      • Stem Cell Culture
      Biological Information
      Gene Symbol
      • RSPO1
      Media FormLiquid
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Appearance (color): clear, red liquid
      • Endotoxin: <2 EU/mL
      • Sterility Tested: No Growth/Pass
      • Mycoplasma: Negative
      • Media are tested for function in supporting growth of human intestinal organoids
      • Media are tested for levels of RSPO-1 by ELISA
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRSPO-1 Conditioned Medium should be stored at -20°C. When ready to use, thaw the supplement overnight at 2-8°C in the dark. Thawed RSPO-1 Conditioned Medium is good for two weeks when stored at 2-8°C.
      Packaging Information
      Material Size50 mL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCM104 04054839008368