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MABF2771-100UL Anti-564/idiotype Antibody, clone 9D11

Anti-564/idiotype, clone 9D11, Cat. No. MABF2771, is a mouse monoclonal antibody that detects mouse IgG idiotype and is tested in Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, , Immunoprecipitation, & Western Blotting.

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MABF2771-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2771-100UL
      DescriptionAnti-564/idiotype Antibody, clone 9D11
      Alternate Names
      • Idiotype
      Background InformationAn idiotype is the genetically determined variation of structures in the variable regions of immunoglobulins. Anti-idiotypic antibodies are known to target specific epitopes, on another antibody that are unique for that target antibody. The collection of epitopes for a given antibody defines its idiotype and is generally found in the complementarity determining regions of that targeted antibody. These antibodies can also bind shared epitopes on highly related monoclonal antibodies. Their specificity allows for the specific detection of antibody of interest even in the presence of endogenous polyclonal antibody. Due to their specificity for the variable region, these antibodies can also be useful in pharmacokinetic and immunogenicity studies. 564Igi is shown to be a reliable murine model of systemic lupus erythematous (SLE). These mice develop SLE profile closely resembling that of human SLE. These mice possess rearranged immunoglobulin heavy- and light-chain genes inserted into their respective loci that are together specific for nucleolar antigens. The 564 antibody is reported to have a characteristic idiotype and B cells carrying the corresponding B-cell antigen receptor are idiotype positive. The epitope recognized by the 564 idiotype antibody is a domain that may be common to multiple self-antigens. (Ref.: Berland, R., et al. (2006). Immunity 25(3); 429-440).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-564/idiotype, clone 9D11, Cat. No. MABF2771, is a mouse monoclonal antibody that detects mouse IgG idiotype and is tested in Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, , Immunoprecipitation, & Western Blotting.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesTested Applications

      Flow Cytometry Analysis: A representative lot detected 564/idiotype in Flow Cytometry applications (Chatterjee, P., et al. (2013). Eur J Immunol. 43(9):2441-2450; van der Poel, C.E., et al. (2019). Cell Rep. 29(9):2745-2755.e4; Simoni, L., et al. (2020). Cell Rep. 33(5):108330).

      Immunohistochemistry Applications: A representative lot detected 564/idiotype in Immunohistochemistry applications (Chatterjee, P., et al. (2013). Eur J Immunol. 43(9):2441-2450).

      Immunoprecipitation Analysis: A representative lot detected 564/idiotype in Immunoprecipitation applications (Chatterjee, P., et al. (2013). Eur J Immunol. 43(9):2441-2450).

      Immunocytochemistry Analysis: A representative lot detected 564/idiotype in Immunocytochemistry applications (Chatterjee, P., et al. (2013). Eur J Immunol. 43(9):2441-2450).

      Immunofluorescence Analysis: A representative lot detected 564/idiotype in Immunofluorescence applications (Simoni, L., et al. (2020). Cell Rep. 33(5):108330).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenAn idiotype of a mouse IgG (clone C11) representing B cell receptor expressed by auto reactive B cells in 564 Igi mice.
      EpitopeUnknown
      Clone9D11
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 9D11 is a mouse monoclonal antibody that detects idiotype of a mouse IgG.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~54 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Idiotype of a mouse IgG target antigen (clone C11).

      Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected idiotype of a mouse IgG target antigen (clone C11) .
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2771-100UL 04065267613987

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