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05-1427

Anti-ARF1 Antibody, clone 3F1

clone 3F1, from mouse

Synonym(s):

ADP-ribosylation factor 1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
3F1, monoclonal
Application:
ELISA, IHC, WB
Citations:
1
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biological source

mouse

Quality Segment

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3F1, monoclonal

species reactivity

rat, human, mouse

technique(s)

ELISA: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

isotype

IgG2a

NCBI accession no.

NM_001024226.1

UniProt accession no.

P84077

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ARF1(375)
mouse ... Arf1(11840)

General description

ARF1 is a member of the ARF family of small G proteins and is a component of Golgi-derived non-clathrin coated vesicles. This antibody is class 1 ARF specific.
~21 kDa

Immunogen

Synthetic peptide corresponding to the C-terminal amino acid sequence of human ARF1.

Application

Anti-ARF1 Antibody, clone 3F1 detects level of ARF1 & has been published & validated for use in WB, ELISA, IH(P).
ELISA: A previous lot of this antibody was used in ELISA.
Optimal working dilutions must be determined by end user.

Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of ARF1 Monoclonal Antibody: Skin & Breast Cancer

Biochem/physiol Actions

Recognizes human ARF1

Physical form

Format: Purified
Purified mouse monoclonal IgG2a in 0.02 M phosphate buffer, 0.25 M NaCl, pH 7.6, with 0.1% sodium azide

Analysis Note

Routinely evaluated by Western Blot on HeLa lysates.

Western Blot Analysis: 1:500 dilution of this lot detected ARF1 on 10 μg of HeLa lysates.

Other Notes

Replaces: MAB3779

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

05-1427:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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