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ABS2247-25UG Anti-Acetyl-Miro1 (Lys 105)

Anti-Acetyl-Miro1 (Lys 105), Cat. No. ABS2247, is a rabbit polyclonal antibody that detects mitochondrial Rho GTPase (Miro) acetylated on Lysine 105 and is tested for use in ELISA, Immunofluorescence, and Western Blotting.

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ABS2247-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABS2247-25UG
      DescriptionAnti-Acetyl-Miro1 (Lys 105)
      Alternate Names
      • Mitochondrial Rho GTPase
      Background InformationMitochondrial Rho GTPase (UniProt: A1L1L6; also known as Miro1) is encoded by the Rhot1 (also known as Rnf135, rCG34120) gene (Gene ID: 303351) in rat. Miro1 is a single-pass type IV membrane protein with a transmembrane domain (aa 605-627). It functions as a mitochondrial GTPase and is involved in mitochondrial trafficking and is considered to be a potential adaptor for microtubule‐based peroxisome motility in mammalian cells. It plays a key role in mitochondrial motility on microtubules and in homeostasis. In mammals, Miro1 and Miro2 share about 60% sequence homology. Three acetylation sites for Miro1 have been identified in rat that are located on lysines 105, 525, and 629. Acetylation of Miro1 on lysine 105 (K105) is shown to increase mitochondrial transport and axon growth. Exposure of neurons to myelin-associated glycoprotein (MAG) and chondroitin sulfate proteoglycans reduces K105 acetylation of Miro1, which reduces axonal mitochondrial transport. Ac-Miro1 undergoes deacetylation with histone deacetylase 6 (HDAC6) and inhibition of HDAC6 increases Miro1 acetylation in axons. Loss of Miro1 in neurons causes depletion of mitochondria from corticospinal tract axons and leads to progressive neurological deficits similar to upper motor neuron disease. (Ref.: Kalinkski, AL., et al. (2019). J. Cell Biol. 218(6); 1871-1890; Castro, IG., et al. (2018). Traffic. 19(3); 229-242; Nguyen, TT., et al. (2014). Proc. Natl. Acad. Sci. USA. 111(35); E3631-E3640).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Acetyl-Miro1 (Lys 105), Cat. No. ABS2247, is a rabbit polyclonal antibody that detects mitochondrial Rho GTPase (Miro) acetylated on Lysine 105 and is tested for use in ELISA, Immunofluorescence, and Western Blotting.
      Key Applications
      • Western Blotting
      • Enzyme Immunoassay (ELISA)
      • Immunofluorescence
      Application NotesTested Applications
      Evaluated by Western Blotting in Rat cortical neurons treated with Tubastatin A.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected lysine 105 acetylated Mitochondrial Rho GTPase (Miro) in lysate from rat cortical neurons treated with Tubastatin A (10 µM; 4 h)
      Immunofluorescence Analysis: A representative lot detected Acetyl-Miro1 (Lys 105) in Immunofluorescence applications (Kalinski, A., et. al. (2019). J Cell Biol. 218(6):1871-1890).
      Western Blotting Analysis: A representative lot detected Acetyl-Miro1 (Lys 105) in Western Blotting applications (Kalinski, A., et. al. (2019). J Cell Biol. 218(6):1871-1890).
      ELISA Analysis: A representative lot detected Acetyl-Miro1 (Lys 105) in ELISA applications (Kalinski, A., et. al. (2019). J Cell Biol. 218(6):1871-1890).
      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenLinear peptide corresponding to 10 amino acids surrounding acetylated lysine 105 from the N-terminal half of rat Mitochondrial Rho GTPase (Miro1).
      EpitopeN-terminal half
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects mitochondrial Rho GTPase acetylated on lysine 105. It targets an epitope within 10 amino acids surrounding acetylated lysine 105 from the N-terminal half.
      IsotypeIgG
      Species Reactivity
      • Rat
      Species Reactivity NoteRat. Predicted to react with Human, Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Rhot1
      • Rnf135
      • rCG34120
      Modifications
      • Acetylation
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~54 kDa observed; 72.16 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Rat cortical neurons treated with Tubastatin A.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected lysine 105 acetylated Mitochondrial Rho GTPase (Miro) in lysate from rat cortical neurons treated with Tubastatin A (10 µM; 4 h)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C . Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS2247-25UG 04065266298222