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MABT188 Anti-Dynamin-1/2 Antibody, clone Hudy-1

Anti-Dynamin-1/2 Antibody, clone Hudy-1 detects level of Dynamin-1/2 & has been published & validated for use in Western Blotting, Immunofluorescence.

Anti-Dynamin-1/2 Antibody, clone Hudy-1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: DNM1, DNM UniProt Number: Q05193
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MABT188
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, HWB, IFMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT188
      DescriptionAnti-Dynamin-1/2 Antibody, clone Hudy-1
      Alternate Names
      • Dynamin-1
      • Dynamin
      • Dynamin-1/2
      Background InformationDynamin-1 & Dynamin-2 belong to the dynamin superfamily of large GTPases. Dynamins are multidomain proteins consisting of a GTP-binding domain, a middle domain, and a GTP-effector domain. Dynamins also contain a pleckstrin homology (PH) domain which may facilitate membrane binding. Dynamin-1 is highly abundant in the brain particularly at synaptic contacts, whereas Dynamin-2 is ubiquitously expressed in non-neuronal cells. Dynamins are involved in receptor-mediated endocytosis of clathrin-coated vesicles. Dynamin-1 interacts with an array of cellular targets via its SH3 binding domain and is implicated in late stages of vesicle formation by forming oligomers around the “neck” of budding vesicles and inducing membrane fission; it may also play a role in cytoskeleton rearrangement and cytoskinesis. Dynamin-2 is involved in the formation of clathrin-coated vesicles but may also be involved in the recycling of receptors between endosomes and the trans-Golgi network.
      References
      Product Information
      FormatPurified
      Control
      • Rat brain tissue lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4) and 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Dynamin-1/2 Antibody, clone Hudy-1 detects level of Dynamin-1/2 & has been published & validated for use in Western Blotting, Immunofluorescence.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesImmunofluorescence Analysis: A representative lot was used by an independent laboratory in HeLa cells (Nicoziani, P., et al. (1999). Molecular Biology of the Cell. 11:481–495.)
      Biological Information
      ImmunogenRecombinant full length human Dynamin-1 expressed in transfected Sf9 cells.
      CloneHudy-1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody detects both Dynamin-1 and Dynamin-2. (Nicoziani, P., et al. (1999). Molecular Biology of the Cell. 11:481–495.)
      IsotypeIgG1κ
      Species Reactivity
      • Rat
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008].
      Gene Symbol
      • DNM1
      • DNM
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis.

      CATALYTIC ACTIVITY: GTP + H2O = GDP + phosphate.

      SUBUNIT STRUCTURE: Interacts with CAV1 and SH3GLB1. Binds SH3GL1, SH3GL2 and SH3GL3 (By similarity). Interacts with PHOCN By similarity. Interacts with SNX9.

      SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm › cytoskeleton. Note: Microtubule-associated.

      SEQUENCE SIMILARITIES: Belongs to the dynamin family.

      Contains 1 GED domain.

      Contains 1 PH domain.
      Molecular Weight~97 kDa observed.
      Dynamin 1 has a calculated MW of 97 kDa. Dynamin 2 has a calcluated MW of 98 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in rat brain tissue lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected Dynamin-1/2 on 10 µg of rat brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT188 04053252548925

      Documentation

      Anti-Dynamin-1/2 Antibody, clone Hudy-1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Dynamin-1/2 Antibody, clone Hudy-1 Certificates of Analysis

      TitleLot Number
      Anti-Dynamin-1/2, clone Hudy-1 3073853
      Anti-Dynamin-1/2, clone Hudy-1 - 2061574 2061574
      Anti-Dynamin-1/2, clone Hudy-1 - 2829145 2829145
      Anti-Dynamin-1/2, clone Hudy-1 - 3202280 3202280
      Anti-Dynamin-1/2, clone Hudy-1 - 3314355 3314355
      Anti-Dynamin-1/2, clone Hudy-1 - 4020098 4020098
      Anti-Dynamin-1/2, clone Hudy-1 - 4142790 4142790
      Anti-Dynamin-1/2, clone Hudy-1 - Q1984917 Q1984917
      Anti-Dynamin-1/2, clone Hudy-1 -2502608 2502608
      Anti-Dynamin-1/2, clone Hudy-1 -2571280 2571280
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