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MABE1881-25UL Anti-EBNA-1 Antibody, clone 1EB12

Anti-EBNA-1, clone 1EB12, Cat. No. MABE1881, is a mouse monoclonal antibody that detects Epstein-Barr nuclear antigen 1 and is tested for use in ELISA, Immunoprecipitation, and Western Blotting.

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MABE1881-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABE1881-25UL
      DescriptionAnti-EBNA-1 Antibody, clone 1EB12
      Alternate Names
      • Epstein-Barr nuclear antigen 1
      • EBV nuclear antigen 1
      Background InformationEpstein-Barr nuclear antigen 1 (UniProt: P03211; also known as EBNA-1, EBV nuclear antigen 1) is encoded by the EBNA1 (also known as BKRF1) gene (Gene ID: 3783709) in Epstein-Barr virus (EBV). EBV is a gamma-herpesvirus, a causative agent of infectious mononucleosis. EBNA1, a multi-functional protein, plays an important role in maintaining EBV in the host cell and has been implicated in host cell immortalization. It plays an essential role in replication and partitioning of viral genomic DNA during latent viral infection. During this phase, the circular double-stranded viral DNA undergoes replication once per cell cycle and is efficiently partitioned to the daughter cells. EBNA1 activates the initiation of viral DNA replication through binding to specific sites in the viral latent origin of replication, oriP. It is also reported to direct the segregation of viral episomes by mediating their attachment to host cell metaphase chromosomes and is known to activate the transcription of several viral latency genes. By counteracting the stabilization of host p53, it also reduces apoptosis and increases host cell survival. EBNA1 is also a risk factor in a variety of malignancies and is the only viral protein found in all EBV-related malignancies. Its C-terminal region contains a dimerization domain and a DNA binding domain (aa 459-607). Although this domain is essential for its replication function of EBNA1, it is not sufficient for this activity and the N-terminal half of EBNA1 is also required. (Ref.: Sun, X., et al. (2010). Proc. Natl. Acad. Sci. USA. 107(7); 3146-3151; Duellman, SJ., and Burgess, RR. (2009). PLoS ONE 4(2): e4614; Wu, H., et al. (2002). J. Virol. 76(5); 2480-2490).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-EBNA-1, clone 1EB12, Cat. No. MABE1881, is a mouse monoclonal antibody that detects Epstein-Barr nuclear antigen 1 and is tested for use in ELISA, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • ELISA
      Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated EBNA-1 in Immunoprecipitation application. (Duellman, S.J., et al. (2009). PLoS One. 4(2):e4614; Sun, X., et al. (2010). Proc Natl Acad Sci USA. 107(7):3146-51).

      ELISA Analysis: A representative lot detected EBNA-1 in ELISA application. (Duellman, S.J., et al. (2009). PLoS One. 4(2):e4614).

      Western Blotting Analysis: A representative lot detected EBNA-1 in Western Blotting application. (Duellman, S.J., et al. (2009). PLoS One. 4(2):e4614; Sun, X., et al. (2010). Proc Natl Acad Sci USA. 107(7):3146-51).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged recombinant Epstein-Barr nuclear antigen 1 expressed in E. coli.
      EpitopeN-terminal
      Clone1EB12
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 1EB12 is a mouse monoclonal antibody that detects Epstein-Barr nuclear antigen 1 (EBNA-1). It targets an epitope within 26 amino acids from the N-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Virus
      Species Reactivity NoteVirus.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • EBNA1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~56 kDa observed; 56.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Raji cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected EBNA-1 in Raji cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1881-25UL 04065266876314