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MABN448

Anti-ENC-1 Antibody, clone 1H8.1

clone 1H8.1, from mouse

Synonym(s):

Ectoderm-neural cortex protein 1, ENC-1, Kelch-like protein 37, Nuclear matrix protein NRP/B, p53-induced gene 10 protein

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
1H8.1, monoclonal
Application:
IHC, WB
Citations:
-
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biological source

mouse

Quality Segment

100

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

1H8.1, monoclonal

species reactivity

human, rat

technique(s)

immunohistochemistry: suitable, western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_001243503

UniProt accession no.

O14682

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ENC1(8507)

General description

Ectoderm-neural cortex protein 1 (ENC1) as its name suggests is a protein involved in the regulation and differentiation of neural crest cells and neuronal development, as well as a protein involved in neuronal process formation later on. It is also known as nuclear matrix protein NRP/B. It is an actin binding protein that appears important in the organization of the actin cytoskeleton during neural fate specification and nervous system development. Its role in neuronal process formation is via its binding of RB1 (p110RB) when it is hypophosophorylated. ENC1 is widely expressed during development in the fetal heart, lung, and kidney. In the adult ENC1 is more restricted to nervous tissues primarily. It is abundant in adult brain, hippocampus, amygdale and spinal cord. There is also expression found in the adult pancreas. Numerous neuronal cancer cell lines and primary brain tumors also express ENC1. Localization is nuclear for the most part but in some cancer cells cytoplasmic staining is also observed. Its role in tumors is as an oncogene as studies suggest that both amplification and alteration of ENC1 may lead to tumor development and growth. Moreover ENC1 plays a role in the oxidative stress response as a regulator of the transcription factor Nrf2, and expression of this gene may play a role in malignant transformation as well.
~66 kDa observed

Immunogen

GST-tagged recombinant protein corresponding to human ENC-1.

Application

Anti-ENC-1 Antibody, clone 1H8.1 is a highly specific mouse monoclonal antibody, that targets Ectoderm-neural cortex-1 & has been tested in western blotting & IHC.
Western Blotting Analysis: 1 µg/mL from a representative lot detected ENC-1 in 10 µg of rat hippocampus tissue.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected ENC-1 in human cerebellum and cerebral cortex tissue.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in human hippocampus tissue.

Western Blotting Analysis: 1 µg/mL of this antibody detected ENC-1 in 10 µg of human hippocampus tissue.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MABC685

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

MABN448:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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