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MABC681

Anti-ETV4 Antibody, clone 1A2G3

ascites fluid, clone 1A2G3, from mouse

Synonym(s):

ETS translocation variant 4, Adenovirus E1A enhancer-binding protein, E1A-F, Polyomavirus enhancer activator 3 homolog, Protein PEA3

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
1A2G3, monoclonal
Application:
WB
Citations:
-
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biological source

mouse

Quality Segment

100

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

1A2G3, monoclonal

species reactivity

human

technique(s)

western blot: suitable

isotype

IgG1

UniProt accession no.

P43268

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ETV4(2118)

General description

ETS translocation variant 4 protein, alternatively known as Adenovirus E1A enhancer-binding protein E1A-F, or Polyomavirus enhancer activator 3 homolog (PEA3), is encoded by the human gene named ETVA/E1AF/PEA3. ETV4 is a protein that is a transcriptional activator that was first recognized as such because it allowed for the expression of the adenovirus E1A gene upon infection. Today ETV4 has been shown to be active in a wide variety of biological processes from stem cell differentiation, to mammary gland and duct morphogenesis to motor neuron and axon guidance. ETV4 activity is regulated via the ERK/MAP signaling and a complex interplay of post-translation modifications such as sumoylation and ubiquitination. In cancers, ETV4 activates HIF and its subsequent proliferation genes and overexpression in prostate cells leads to oncogenesis. EMD-Millipore’s Anti-ETV4 mouse monoclonal antibody has been tested in western blot against K562 cell lysates as well as recombinantly expressed ETV4 protein.
~54 kDa observed. Uncharacterized bands may appear in some lysate(s).

Immunogen

Purified recombinant fragment of human ETV4 expressed in E. Coli.

Application

Detect ETS translocation variant 4 protein using this mouse monoclonal antibody, Anti-ETV4 Antibody & clone 1A2G3 validated for use in western blotting.

Analysis Note

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ETV4 in K562 cell lysate.

Optimal working dilutions must be determined by end user.

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

MABC681:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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