Skip to Content
Merck

Key Documents

View All Documentation

04-1585

Anti-IL-18 Antibody, clone 12E7.1

clone 12E7.1, from mouse

Synonym(s):

interleukin 18 (interferon-gamma-inducing factor), IL-1 gamma, IFN-gamma-inducing factor, iboctadekin, interleukin-18, Interleukin-1 gamma, Iboctadekin, Interferon gamma-inducing factor

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
12E7.1, monoclonal
Application:
FACS, IHC
Citations:
3
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

mouse

Quality Segment

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

12E7.1, monoclonal

species reactivity

human, mouse

technique(s)

flow cytometry: suitable, immunohistochemistry: suitable

isotype

IgMκ

NCBI accession no.

NP_001553

UniProt accession no.

Q14116

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... IL18(3606)
mouse ... Il18(16173)

General description

Interleukin-18 (UniProt: Q14116; also known as IL-18, Iboctadekin, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma) is encoded by the IL18 (also known as IGIF, IL1F4) gene (Gene ID: 3606) in human. IL-18 is a pro-inflammatory cytokine that is shown to evoke both innate and acquired responses. It is synthesized with a propeptide (aa 1-36), which is subsequently cleaved off by caspases 1 and 4 to generate the active mature form. IL-18 is reported to be constitutively expressed in monocytes, macrophages, epithelial cells, osteoblasts, keratinocytes, and synovial fibroblasts. Its expression has also been reported in adrenal cortical cells. Mature IL-18 acts by binding to the IL-18 receptor α (IL-18α) to form a low affinity binary complex that recruits IL-18β to form a high affinity complex that leads to the activation of NF-kB and MAP kinase pathways. IL-18 can synergize with IL-12 to induce interferon-γ synthesis from T-helper 1 and natural killer cells.
~24 kDa observed

Immunogen

KLH-conjugated linear peptide corresponding to human IL-18.

Application

Anti-IL-18 Antibody, clone 12E7.1 is an antibody against IL-18 for use in IH, FC.
Immunohistochemistry Analysis: 1:250 from a previous lot detected IL-18 in human tonsil tissue.

Immunohistochemistry Analysis: 1:250 from a previous lot detected IL-18 in inflamed skin and normal human spleen tissues.
Research Category
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors

Physical form

Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse splenocytes
Evaluated by Flow Cytometry in mouse splenocytes.

Flow Cytometry Analysis: 1 g of this antibody detected IL-18 in mouse splenocytes.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Still not finding the right product?

Try our Product Selector Tool to narrow your options

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Storage Class

12 - Non Combustible Liquids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

04-1585:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library