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MAB1978-I-100UL Anti-Integrin αV Antibody, clone LM142

Anti-Integrin aV, clone LM142, Cat. No. MAB1978-I, is a mouse monoclonal antibody that detects Integrin aV and is used in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Surface plasmon resonance.

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MAB1978-I-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMAB1978-I-100UL
      DescriptionAnti-Integrin αV Antibody, clone LM142
      Alternate Names
      • Integrin alpha-V
      • Vitronectin receptor
      • Vitronectin receptor subunit alpha
      • CD51
      Background InformationIntegrin aV (UniProt: P06756; also known as Vitronectin receptor, Vitronectin receptor subunit alpha, CD51) is encoded by the ITGAV (also known as MSK8, VNRA, VNTR) gene (Gene ID: 3685) in human. Integrins are heterodimeric integral membrane proteins composed of an a subunit and a b subunit that function in cell surface adhesion and signaling. They contain a large extracellular domain responsible for ligand binding, a single transmembrane domain, and a cytoplasmic domain. The exact combination of various a- and b-subunits dictates the binding specificity of integrins to different ECM components. Although both subunits are required for adhesion, the binding specificity primarily depends on the extracellular portion of the a-subunit. The structural and functional diversity of the integrin family is based upon the pairing abilities of the individual a and b subunits. Integrins are not constitutively active and their activation from a low ligand-binding affinity state to high ligand-binding affinity state requires conformational change that can originate from either from their cytoplasmic or extracellular interactions. The recognition site for most integrins that bind the ECM consists of an RGD (arginine-glycine-aspartic acid) sequence. Integrin aV is a single-pass membrane glycoprotein that is synthesized with a signal peptide (aa 1-30), which is subsequently cleaved off to generate the mature form. The mature form contains an extracellular domain (aa 31-992), a transmembrane domain (aa 993-1016), and a cytoplasmic domain (aa 1017-1048). The a-subunit is composed of a heavy (aa 31-889) and a light (aa 891-1048) chain linked by a disulfide bond. Integrin aV can associate with b-1, b-3, b -5, b-6, or b-8 in a non-covalent manner. (Ref.: Cheresh, DA. (1987). Proc. Natl. Acad. Sci. USA. 84(18); 6471-6475).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Integrin aV, clone LM142, Cat. No. MAB1978-I, is a mouse monoclonal antibody that detects Integrin aV and is used in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Surface plasmon resonance.
      Key Applications
      • Affinity Binding Assay
      • Flow Cytometry
      • Immunocytochemistry
      • Immunofluorescence
      • Immunoprecipitation
      • Surface plasmon resonance
      Application NotesTested Applications

      Surface plasmon resonance: A representative lot detected Integrin aV in Surface plasmon resonance applications (Chesnokova, L.S., et. al. (2011). J Virol. 85(24):13214-23).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated Integrin aV in Immunoprecipitation applications (Ueda, M., et. al. (2010). Biomaterials. 31(25):6394-9; Cheresh, D.A., et. al. (1987). Proc Natl Acad Sci USA. 84(18):6471-5).

      Immunocytochemistry Analysis: A 1:25 dilution from a representative lot detected Integrin aV in A431 cells.

      Immunofluorescence Analysis: A representative lot detected Integrin aV in Immunofluorescence applications (Straub, S., et. al. (2013). PLoS One. 8(1):e53309).

      Affinity Binding Assay: A representative lot detected Integrin aV in Affinity Binding Assay applications (Kapp, T.G., et. al. (2017). Sci Rep. 7:39805).

      Flow Cytometry Analysis: A representative lot detected Integrin aV in Flow Cytometry applications (Borbely, A., et. al. (2019). Pharmaceutics. 11(4):151).

      Immunocytochemistry Analysis: A representative lot detected Integrin aV in Immunocytochemistry applications (Cheresh, D.A., et. al. (1987). Proc Natl Acad Sci USA. 84(18):6471-5).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenPurified aVb3 isolated from human placenta.
      EpitopeUnknown
      CloneLM142
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone LM142 is a mouse monoclonal antibody that detects human Integrin aV.
      IsotypeIgG1
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ITGAV
      • MSK8
      • VNRA
      • VTNR
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight116.04 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in HeLa cells.

      Flow Cytometry Analysis: 1 µg of this antibody detected Integrin aV in one million HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB1978-I-100UL 04065266553932

      Documentation

      Anti-Integrin αV Antibody, clone LM142 Certificates of Analysis

      TitleLot Number
      Anti-Integrin αV, clone LM142 - 3899618 3899618
      Anti-Integrin αV, clone LM142 - 4001212 4001212
      Anti-Integrin αV, clone LM142 - 4001604 4001604
      Anti-Integrin αV, clone LM142 - 4053761 4053761
      Anti-Integrin αV, clone LM142 - 4054123 4054123
      Anti-Integrin αV, clone LM142 - 4056343 4056343
      Anti-Integrin αV, clone LM142 - 4059351 4059351
      Anti-Integrin αV, clone LM142 - 4070803 4070803
      Anti-Integrin αV, clone LM142 - 4078195 4078195
      Anti-Integrin αV, clone LM142 - 4079400 4079400
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