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MAB413-I-25UG Anti-Interferon-α Antibody, clone ST126

Anti-Interferon-a, clone ST126, Cat. No. MAB413-I, is a mouse monoclonal antibody that detects Interferon alpha-2 and is tested for use in ELISA, Immunohistochemistry (Paraffin), Neutralizing, Radioimmunoassay, and Western Blotting.

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MAB413-I-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMAB413-I-25UG
      DescriptionAnti-Interferon-α Antibody, clone ST126
      Alternate Names
      • Interferon alpha-2
      • IFN-alpha-2
      • Interferon alpha-A
      • LeIF A
      Background InformationInterferon alpha-2 (UniProt: P01563; also known as IFN-alpha-2, Interferon alpha-A, LeIF A) is encoded by the IFNA2 (also known as IFNA2A, IFNA2B, IFNA2C) gene (Gene ID: 3440) in human. Interferons are divided into three families (type I, II, and III) based on their sequence homology. Type I is the largest group that includes Interferons a and b are the primary defensive cytokines against pathogens and display strong antiviral activities. They also display anti-bacterial and anti-cancer properties. IFN-a is rapidly induced and secreted by cells to limit virus replication through numerous and varied mechanisms, including the induction of intrinsic restriction factors. IFN-a2 is produced by macrophages with a signal peptide (aa 1-23), which is subsequently cleaved off to generate the mature active form. It is produced in response to viral infection and triggers the induction of protective genes that inhibit viral replication and impede viral dissemination. IFN-a2 is shown to be an effective agent against hepatitis viruses. Chronic administration of IFN-a is shown to cause the regression of vascular neoplasms, including hemangiomas, Kaposi's sarcoma, and pulmonary hemangiomatosis. (Ref.: Lavender, KJ., et al. (2016). J, Virol. 90(13); 6001-6013).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Interferon-a, clone ST126, Cat. No. MAB413-I, is a mouse monoclonal antibody that detects Interferon alpha-2 and is tested for use in ELISA, Immunohistochemistry (Paraffin), Neutralizing, Radioimmunoassay, and Western Blotting.
      Key Applications
      • Western Blotting
      • Neutralizing
      • Radioimmunoassay
      • Immunohistochemistry (Paraffin)
      • ELISA
      Application NotesNeutralizing: A representative lot of this antibody neutralized the antiviral activity of Interferon-a. (Shearer, M., et. al. (1984). J Immunol. 133(6):3096-101).

      ELISA Analysis: A representative lot detected Interferon-a in ELISA applications (Shearer, M., et. al. (1984). J Immunol. 133(6):3096-101).

      Radioimmunoassay: A representative lot detected Interferon-a in Radioimmunoassay applications (Shearer, M., et. al. (1984). J Immunol. 133(6):3096-101).

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Interferon-a in human kidney and human stomach cancer tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenA full-length purified human interferon-a produced by human Burkitt lymphoma Namalwa cell line.
      EpitopeUnknown
      CloneST126
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone ST126 is a mouse monoclonal antibody that detects human Interferon a.
      IsotypeIgG2b
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IFNA2
      • IFNA2A
      • IFNA2B
      • IFNA2C
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~19 kDa observed; 21.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant human IFN- a2.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant human IFN-a2.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB413-I-25UG 04065265944830