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MAB2041-I-100UL Anti-Laminin β1 Antibody, clone 3E5

This mouse monoclonal Anti-Laminin β1, clone 3E5, Cat. No. MAB2041-I is tested for use in Western Blotting and ELISA, Inhibition, and Electron Microscopy, for the detection of Laminin subunit β1.

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MAB2041-I-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMAB2041-I-100UL
      DescriptionAnti-Laminin β1 Antibody, clone 3E5
      Alternate Names
      • Laminin subunit beta-1
      • Laminin B1 chain
      • Laminin-1 subunit beta
      Background InformationLaminin subunit b1 (UniProt: P07942; also known as Laminin B1 chain, Laminin-1 subunit beta) is encoded by the LAMB1 gene (Gene ID: 3912) in human. Laminins are the major non-collagenous constituent of basement membranes that participate in wide variety of biological processes, including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. They are a family of heterotrimeric extracellular glycoproteins consisting of α, β, and γ chains that are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Laminins mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Thus far five α, three β, and three γ subunits are described that assemble into at least 15 different laminin molecules. Different α, β, and γ chain isomers combine to give rise to different heterotrimeric laminin isoforms, which are designated by Arabic numerals in the order of their discovery. Laminin- β1 is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form. It is expressed in most tissues that produce basement membranes and is one of the 3 chains constituting laminin 1. It is shown to be involved in the organization of the laminar architecture of cerebral cortex and is probably required for the integrity of the basement membrane/glia limitans that serves as an anchor point for the end feet of radial glial cells and as a physical barrier to migrating neurons. (Ref.: Radmanesh, F., et al. (2013). Am. J. Hum. Genet. 92(3); 468-474; Virtanen, I., et al. (1995). J. Histochem. Cytochem. 43(6); 621-628)).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationThis mouse monoclonal Anti-Laminin β1, clone 3E5, Cat. No. MAB2041-I is tested for use in Western Blotting and ELISA, Inhibition, and Electron Microscopy, for the detection of Laminin subunit β1.
      Key Applications
      • Western Blotting
      • Inhibition
      • Electron Microscopy
      • ELISA
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected Laminin β1 in Western Blotting applications (Engvall, E., et al. (1986). J Cell Biol.;103(6 Pt1):2457-65).

      Electron Microscopy: A representative lot detected Laminin β1 in Electron Microscopy applications (Engvall, E., et al. (1986). J Cell Biol.;103(6 Pt1):2457-65).

      Inhibition: A representative lot inhibited the neurite-promoting activity of laminin. (Engvall, E., et al. (1986). J Cell Biol.;103(6 Pt1):2457-65).

      ELISA Analysis: A representative lot detected Laminin β1 in ELISA applications (Engvall, E., et al. (1986). J Cell Biol.;103(6 Pt1):2457-65).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenLaminin fragments from human placenta.
      EpitopeUnknown
      Clone3E5
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 3E5 is a mouse monoclonal antibody that detects Laminin subunit β1. It targets an epitope near the end of long arm.
      IsotypeIgG
      Species Reactivity
      • Rat
      • Human
      Species Reactivity NoteHuman, Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LAMB1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~200 kDa observed; 198.04 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Human placenta tissue lysates.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Laminin β1 in Human placenta tissue lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB2041-I-100UL 04065266791471