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MABF2090-100UG Anti-Ly49A Antibody, clone A1

This mouse monoclonal Anti-Ly49A, clone A1, Cat. No. MABF2090 is tested for use in Flow Cytometry, Immunocytochemistry and Immunoprecipitation, for the detection of Lymphocyte antigen 49a.

Anti-Ly49A Antibody, clone A1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: Klra1, LY-49, Ly-49a, LY49A UniProt Number: P20937
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MABF2090-100UG
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MFC, ICC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF2090-100UG
      DescriptionAnti-Ly49A Antibody, clone A1
      Alternate Names
      • T-cell surface glycoprotein YE1/48
      • Lymphocyte antigen 49a
      • Ly-49a
      • T lymphocyte antigen A1
      Background InformationT-cell surface glycoprotein YE1/48 (UniProt: P20937; also known as Lymphocyte antigen 49a, Ly-49a, T lymphocyte antigen A1) is encoded by the Klra1 (also known as Ly-49, Ly-49a, Ly49, Ly49A) gene in murine species. LY49A is a single-pass type II membrane protein that serves as an inhibitory receptor on natural killer (NK) cells for H-2d(Dd) MHC class I molecules on target cells. It inhibits the activity of NK cells thereby preventing cell lysis. Ly49A-mediated NK cell inhibition is shown to be dependent on a lateral binding site, which is located beneath the peptide-binding platform of Dd. Ly49A is reported to contribute to target cell adhesion and accumulate at synapses with Dd-expressing target cells when NK cells themselves lack Dd. In cases where NK cells display Dd expression Ly49A does not contribute to adhesion. Higher expression of LY49A is observed in T-lymphoma cell lines and normal lymphocytes exhibit low expression. LY49A has a cytoplasmic domain (aa 1-44), a transmembrane domain (aa 45-66), and an extracellular domain (aa 67-262). (Ref.: Back, J., et al. (2007). Proc. Natl. Acad. Sci. USA 104(10); 3978 3983).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis mouse monoclonal Anti-Ly49A, clone A1, Cat. No. MABF2090 is tested for use in Flow Cytometry, Immunocytochemistry and Immunoprecipitation, for the detection of Lymphocyte antigen 49a.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: A representative lot detected Ly49A in Immunoprecipitation applications (Nagasawa, R., et. al. (1987). J Immunol. 138(3):815-24; Yokoyama, W.M., et. al. (1989). J Immunol. 143(4):1379-86).

      Immunocytochemistry Analysis: A representative lot detected Ly49A in Immunocytochemistry applications (Nagasawa, R., et. al. (1987). J Immunol. 138(3):815-24; Back, J., et. al. (2007). Proc Natl Acad Sci U S A. 104(10):3978-83).

      Flow Cytometry Analysis: A representative lot detected Ly49A in Flow Cytometry applications (Nagasawa, R., et. al. (1987). J Immunol. 138(3):815-24; Yokoyama, W.M., et. al. (1989). J Immunol. 143(4):1379-86; Back, J., et. al. (2007). Proc Natl Acad Sci U S A. 104(10):3978-83; Silver, E.T., et. al. (2000). J Immunol. 165(4):1771-81).
      Biological Information
      ImmunogenEL-4 T lymphoma cells.
      CloneA1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone A1 specifically detects Lymphocyte antigen 49a (LY49A) in murine species.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Klra1
      • LY-49
      • Ly-49a
      • LY49A
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight30.50 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in EL-4 T lymphoma cells.

      Flow Cytometry Analysis: 1 µg of this antibody detected Ly49A in 1X10E6 EL-4 T lymphoma cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2090-100UG 04054839529597