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ABF210 Anti-MDA5 Antibody

Detect Melanoma differentiation-associated protein 5 using this rabbit polyclonal antibody, Anti-MDA5 Antibody validated for use in western blotting, IHC (Paraffin) & Immunofluorescence.

Anti-MDA5 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: IFIH1 UniProt Number: Q9BYX4
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ABF210
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P), IFRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABF210
      ReplacesMABF198
      DescriptionAnti-MDA5 Antibody
      Alternate Names
      • MDA5 Antibody
      • Melanoma differentiation-associated protein 5
      • Interferon-induced with helicase C domain protein 1
      • IFIH1
      Background InformationMelanoma differentiation-associated protein 5 (MDA5), also known as Interferon-induced helicase C domain-containing protein 1 (IFIH1) is a member of the helicase family (RLR subfamily). MDA5 plays an important role in recognizing various viral components and triggers antiviral responses. When stimulated by dsRNA, MDA5 recruits adaptor protein VISA causing the activation of IRF-3 and NF-κB. MDA5 is widely expressed at low levels with barely detectable levels found in , testis and lung. Mutations in MDA5 have been associated with diabetes mellitus insulin-dependent type 19.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Daudi cell Lysate
      PresentationPurified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Melanoma differentiation-associated protein 5 using this rabbit polyclonal antibody, Anti-MDA5 Antibody validated for use in western blotting, IHC (Paraffin) & Immunofluorescence.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunofluorescence
      Application NotesImmunohistochemistry Analysis: 5 µg/mL from a representative lot detected MDA5 in human lymph node tissue lysate.

      Immunofluorescence Analysis: 20 μg/mL from a representative lot detected MDA5 in human lymph node cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human MDA5.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes the C-terminus of MDA5.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • IFIH1
      Purification MethodAntigen Affinity Purified
      UniProt Number
      Molecular Weight~117 kDa observed. Uncharacterized bands may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Daudi cell lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected MDA5 in 15 µg of Daudi cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABF210 04053252879883