Skip to Content
Merck

Key Documents

View All Documentation

AB8345

Anti-MMP-14 Antibody

serum, Chemicon®

Synonym(s):

MT1-MMP

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA, FACS, ICC, IP, WB
Citations:
19
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

rabbit

Quality Segment

100

conjugate

unconjugated

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

NCBI accession no.

NM_004995.2

UniProt accession no.

P50281

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... MMP14(4323)

Immunogen

Catalytic domain of MT1-MMP

Application

Anti-MMP-14 Antibody detects level of MMP-14 & has been published & validated for use in ELISA, FC, IP, WB & IC.
Immunocytochemistry: 5-10 μg / ml

Western blot: 5 μg / ml Expected band sizes: 60-63 kDA and 42 kDa pro-enzyme and degradation form respectively.

Immunopreciptation: 1-2 μg / mg total protein in 500 μl reaction volume.

FACS Analysis: 5 μg/ mL (1 million cells per mL)

EIA: 1:10,000

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
MMPs & TIMPs

Biochem/physiol Actions

The antibody recognizes native and recombinant proteins.

Physical form

Liquid in PBS, pH 7.6, containing 0.01% sodium azide as a preservative.

Preparation Note

Store antibody at -20°C for 12 months. Avoid repeat freeze thaw cycles.

Analysis Note

Control
Immunocytochemistry positive control: U251 glioma cells; negative control MCF7 breast carcinoma.

Western Blot positive control: HT1080 fibrosarcoma, negative control: MCF7 breast carcinoma

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Still not finding the right product?

Try our Product Selector Tool to narrow your options

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Storage Class

12 - Non Combustible Liquids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

AB8345:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library