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MABT849-25UG Anti-Myosin 7 (MYH7) Antibody, clone 4B51E8

Anti-Myosin 7 (MYH7), clone 4B51E8, Cat. No. MABT849, is a rat monoclonal antibody that detects Mysoin-7 and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

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MABT849-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABT849-25UG
      DescriptionAnti-Myosin 7 (MYH7) Antibody, clone 4B51E8
      Alternate Names
      • Myosin heavy chain 7
      • Myosin heavy chain slow isoform
      • MyHC-slow
      • Myosin heavy chain
      • Cardiac muscle beta isoform
      • MyHC-beta)
      Background InformationMyosin-7 (UniProt: Q91Z83; also known as Myosin heavy chain 7, Myosin heavy chain slow isoform, MyHC-slow, Myosin heavy chain, cardiac muscle beta isoform, MyHC-beta) is encoded by the MYH7 gene (Gene ID: 140781) in murine species. Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Myosins form regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle. Myosin-7 is a component of thick filaments of the myofibrils. It contains actin- and ATP-binding sites in its conserved catalytic head domain. Its actin binding region is localized to amino acids 655-677 and 757-771 and the ATP-binding region is localized to amino acids 178-185. Myosin-7 has an N-terminal SH3-like domain (aa 32-81), a myosin motor domain (aa 85-778), and an IQ domain (aa 781-810). Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Limited proteolysis of myosin heavy chain produces one light meromyosin (LMM) and one heavy meromyosin (HMM). HMM can be further cleaved into two globular sub fragments (S1) and 1 rod-shaped sub fragment (S2).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Myosin 7 (MYH7), clone 4B51E8, Cat. No. MABT849, is a rat monoclonal antibody that detects Mysoin-7 and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:25 dilution from a representative lot detected Myosin 7 (MYH7) in mouse skeletal muscle tissue.

      Immunohistochemistry Analysis: A representative lot detected Myosin 7 (MYH7) in Immunohistochemistry applications (Sawano, S., et. al. (2016). PLoS One. 11(11):e0166080).

      Immunofluorescence Analysis: A representative lot detected Myosin 7 (MYH7) in Immunofluorescence applications (Sawano, S., et. al. (2016). PLoS One. 11(11):e0166080).

      Western Blotting Analysis: A representative lot detected Myosin 7 (MYH7) in Western Blotting applications (Sawano, S., et. al. (2016). PLoS One. 11(11):e0166080).

      Western Blotting Analysis: 2 µg/mL from a representative lot detected Myosin 7 (MYH7) in mouse soleus muscle tissue lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 39 amino acids from the C-terminal region of murine Mysoin-7.
      Clone4B51E8
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 4B51E8 is a rat monoclonal antibody that detects murine Myosin-7. It targets an epitope within 39 amino acids from the C-terminall half.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Human, Rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Myh7
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight222.88 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in mouse heart tissue.

      Immunohistochemistry (Paraffin) Analysis: A 1:25 dilution of this antibody detected Myosin 7 (MYH7) in mouse heart tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from the date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT849-25UG 04054839500381

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