Millipore Sigma Vibrant Logo

MABC1235 Anti-N-Myc Interactor Antibody, clone 16E9.2

Detect N-Myc Interactor using this mouse monoclonal Anti-N-Myc Interactor Antibody, clone 16E9.2, Cat. No. MABC1235, validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Anti-N-Myc Interactor Antibody, clone 16E9.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: NMI UniProt Number: Q13287
View This Product on Sigma-Aldrich
MABC1235
100 μg  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Special Offers

      100% Performance Guaranteed

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1235
      DescriptionAnti-N-Myc Interactor Antibody, clone 16E9.2
      Alternate Names
      • N-myc-interactor
      • N-myc and STAT interactor
      • Nmi
      Background InformationN-myc-interactor (UniProt Q13287; also known as N-myc and STAT interactor, Nmi) is encoded by the NMI gene (Gene ID 9111) in human. Nmi is expressed in all adult and fetal tissues except brain and skin and is shown to be more abundant in fetal liver. It interacts with all STATs, except STAT2 and augments STAT-mediated transcription in response to IL-2 and gamma-interferon. Interestingly, Nmi lacks an intrinsic transcriptional activation domain; instead, it enhances the association of CBP/p300 coactivator proteins with STAT1 and STAT5. Higher expression of Nmi has been reported in myeloid leukemia that also express higher levels of c-Myc. Ref.: Zhu, M., et al. (1999). Cell 96, 121-130.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect N-Myc Interactor using this mouse monoclonal Anti-N-Myc Interactor Antibody, clone 16E9.2, Cat. No. MABC1235, validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected N-Myc Interactor in 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized HeLa and A431 cells.

      Immunocytochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected N-Myc Interactor in human cerebellum, small instestine, and tonsil tissue sections.
      Biological Information
      ImmunogenGST-tagged recombinant human N-Myc Interactor N-terminal fragment.
      Clone16E9.2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 16E9.2 targeted epitope resides within the N-terminal half of N-Myc Interactor.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NMI
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~39 kDa observed. 35.06 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in K562 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected N-Myc Interactor in 10 µg of K562 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1235 04054839105470