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MABF2237-100UL Anti-NKp30 (CD337) Antibody, clone 1C01

Anti-NKp30 (CD337), clone 1C01, Cat. No. MABF2237, is a This mouse monoclonal antibody that detects NKp30 (Cd337) and is tested for use in Western Blotting, Flow Cytometry, Function Analysis, Immunocytochemistry, and Inhibits Acitivty/Function.

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MABF2237-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2237-100UL
      DescriptionAnti-NKp30 (CD337) Antibody, clone 1C01
      Alternate Names
      • Natural cytotoxicity triggering receptor 3
      • Activating natural killer receptor p30
      • Natural killer cell p30-related protein
      • NK-p30
      • CD337
      Background InformationNatural cytotoxicity triggering receptor 3 (UniProt: O14931; also known as Activating natural killer receptor p30, Natural killer cell p30-related protein, NK-p30, NKp30, CD337) is encoded by the NCR3 (also known as 1C7, LY117) gene (Gene ID: 259197) in human. NKp30 is a single-pass type I membrane protein that is selectively expressed by all resting and activated NK cells. Its weaker expression is observed in spleen. It serves as a receptor of natural killer/NK cell. It is activated by binding of extracellular ligands including BAG6 and NCR3LG1. It stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It cooperates with NKp46 and/or NKp44 in inducing NK-mediated cytotoxicity against the majority of target cells. It is also reported to control NK cells cytotoxicity against tumor cells. Its interaction with BAG6 promotes myeloid dendritic cells (DC) maturation, both through killing DCs that fail to achieve a mature phenotype and by inducing the release of TNF-alpha and interferon-gamma by NK cells that promote DC maturation. NKp30 is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 19-135), a transmembrane domain (aa 136-156), and a cytoplasmic domain (aa 157-201). Six isoforms of NKp30 have been described that are produced by alternative splicing.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-NKp30 (CD337), clone 1C01, Cat. No. MABF2237, is a This mouse monoclonal antibody that detects NKp30 (Cd337) and is tested for use in Western Blotting, Flow Cytometry, Function Analysis, Immunocytochemistry, and Inhibits Acitivty/Function.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Affects Function
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected NKp30 (CD337) in Human tonsil tissue lysate.

      Function Analysis: A representative lot of this antibody blocked the binding of NKp30-Fc to β-1,3-Glucan and reduced Nkp30 clustering. (Li, S.S., et. al. (2018). Nat Commun. 9(1):751; Li, S.S., et. al. (2013). Cell Host Microbe. 14(4):387-97).

      Western Blotting Analysis: A representative lot detected NKp30 (CD337) in Western Blotting applications (Li, S.S., et. al. (2018). Nat Commun. 9(1):751; Li, S.S., et. al. (2013). Cell Host Microbe. 14(4):387-97).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated NKp30 (CD337) in Immunoprecipitation applications ( Li, S.S., et. al. (2013). Cell Host Microbe. 14(4):387-97 ).

      Flow Cytometry Analysis: A representative lot detected NKp30 (CD337) in Flow Cytometry applications (Li, S.S., et. al. (2018). Nat Commun. 9(1):751; Li, S.S., et. al. (2013). Cell Host Microbe. 14(4):387-97).

      Immunocytochemistry Analysis: A representative lot detected NKp30 (CD337) in Immunocytochemitry applications (Li, S.S., et. al. (2013). Cell Host Microbe. 14(4):387-97).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenYT cells
      EpitopeExtracellular domain
      Clone1C01
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 1C01 is a mouse monoclonal antibody that detects human Natural cytotoxicity triggering receptor 3 (NKp30).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NCR3
      • IC7
      • LY117
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~32 kDa observed; 21.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Human spleen tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NKp30 (CD337) in Human spleen tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2237-100UL 04065265651479