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MABE343-AF647

Anti-Puromycin, clone 12D10, Alexa Fluor™ 647 Conjugate Antibody

clone 12D10, 0.5 mg/mL, from mouse

Synonym(s):

Alexa Fluor 647 Anti-Puromycin, Clone 12D10 Anti-Puromycin, Puromycin Detection Antibody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
ALEXA FLUOR™ 647
Clone:
12D10, monoclonal
Application:
ICC
Citations:
17
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biological source

mouse

Quality Segment

100

conjugate

ALEXA FLUOR™ 647

antibody form

purified antibody

antibody product type

primary antibodies

clone

12D10, monoclonal

species reactivity

human

concentration

0.5 mg/mL

technique(s)

immunocytochemistry: suitable

shipped in

wet ice

target post-translational modification

unmodified

General description

Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3′ end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
Refer to Cat. No. MABE343 for observed molecular weight information.
This Anti-Puromycin, clone 12D10 is validated for use in Immunocytochemistry for the detection of Puromycin.

Biochem/physiol Actions

Predicted to react with cells from all species when pre-incubated with puromycin.

Analysis Note

Evaluated by Immunocytochemistry Analysis untreated and puromycin treated HeLa cells.

Immunocytochemistry Analysis: A 1:2,500 dilution of this antibody detected puromycin-incorporated neosynthesized proteins in puromycin treated HeLa cells.

Alexa Fluor™ is a registered trademark of Life Technologies.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

MABE343-AF647:

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Certificates of Analysis (COA)

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