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05-1471

Anti-Rictor Antibody, clone 9F1.2

clone 9F1.2, from mouse

Synonym(s):

Rictor, AVO3, rapamycin-insensitive companion of mTOR, mAVO3

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
9F1.2, monoclonal
Application:
IP, WB
Citations:
6
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biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9F1.2, monoclonal

species reactivity

human, mouse, rat

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_689969.2

UniProt accession no.

Q6R327

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... RICTOR(253260)

General description

190 kDa Observed
Rictor, the rapamycin-insensitive complex, is part of the mTORC2 kinase complex which is responsible for phosphorylating the hydrophobic motif kinase of Akt/PKB. This complex also includes mTOR, mSin1 and mLST8/GL. mTORC2 is activated by growth factors and other mitogenic signals.

Immunogen

Recombinant protein corresponding to human Rictor.

Application

Anti-Rictor Antibody, clone 9F1.2 detects level of Rictor & has been published & validated for use in WB & IP.
Immunoprecipitation:
Representative lot data.
1 ug/mL dilution of this antibody immunoprecipitated Rictor from 100 mg of HEK293 lysate.

Biochem/physiol Actions

This antibody recognizes Rictor.

Physical form

Format: Purified
Purified immunoglobulin in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Analysis Note

Control
HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Rictor on 10 µg of HEK293 cell lysate.
Evaluated by Western Blotting on 10 mg of HEK293 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

05-1471:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Zheng Wang et al.
Neoplasia (New York, N.Y.), 23(11), 1147-1165 (2021-10-28)
Epithelial-mesenchymal transition (EMT) contributes to tumor invasion, metastasis and drug resistance. AKT activation is key in a number of cellular processes. While many positive regulators for either EMT or AKT activation have been reported, few negative regulators are established. Through
Sharon K Kuss-Duerkop et al.
PLoS pathogens, 13(9), e1006635-e1006635 (2017-09-28)
Influenza A virus usurps host signaling factors to regulate its replication. One example is mTOR, a cellular regulator of protein synthesis, growth and motility. While the role of mTORC1 in viral infection has been studied, the mechanisms that induce mTORC1
Mahefatiana Andrianifahanana et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 30(11), 3733-3744 (2016-11-03)
TGF-β plays a central role in the pathogenesis of fibroproliferative disorders. Defining the exact underlying molecular basis is therefore critical for the development of viable therapeutic strategies. Here, we show that expression of the facilitative glucose transporter 1 (GLUT1) is
View All Related Papers

Global Trade Item Number

SKUGTIN
05-147104053252623875