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MAB1098

Anti-Tumor Necrosis Factor-β Antibody, clone 9B9

clone 9B9, Chemicon®, from mouse

Synonym(s):

TNF-beta

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
9B9, monoclonal
Application:
ICC, IHC, WB
Citations:
4
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biological source

mouse

Quality Segment

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9B9, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

isotype

IgG1

NCBI accession no.

NM_000595.2

UniProt accession no.

P01374

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... LTA(4049)

Immunogen

Human, recombinant tumor necrosis factor-beta (TNF-beta, lymphotoxin) produced in E. Coli.

Application

Anti-Tumor Necrosis Factor-β Antibody, clone 9B9 detects level of Tumor Necrosis Factor-β & has been published & validated for use in IC, IH & WB.
Western blot

Immunocytochemistry

Immunohistochemistry

Neutralization: 200ng of TNF-beta antibody is required for the neutralizaion of 1 unit/ml TNF-beta. The TNF-beta-preparation which is used has a specific activity of ca. 2x10(7) units/mg protein [cell lytic assay with L 929 cells (mouse transformed fibroblasts) and/or WEHJ 164 cells (mouse fibrosarcoma cells)/actinomycin D] .

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

This antibody is use for detection, identification and neutralixzation of TNF-b in cell culture and other biological samples. The antibody reacts with human natural and recombinant TNF-b (lymphotoxin). It does not react with human TNF-a. Determination of cross-reactivity to TNF-b from other species has not been done.

Physical form

Format: Purified
Purified immunoglobulin (Protein A-agarose chormotography). One bottle of lyophilized preparation contains:200ug mouse IgG, 2 mg potassium phosphate buffer, 2.9 mg sodium chloride, and 20 mg raffinose.Reconstitution with 1 ml sterile, distilled water results in an antibody concentration of 200 ug/ml.

Preparation Note

Maintain frozen at -20°C in undiluted aliquots for up to 12 months.Freeze only once.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

MAB1098:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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