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CBL1300

Anti-VCAM-1 Antibody, clone M/K

clone M/K - 2, Chemicon®, from rat

Synonym(s):

CD106

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
M/K - 2, monoclonal
Application:
FACS, IHC, IP
Citations:
8
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biological source

rat

Quality Segment

100

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

M/K - 2, monoclonal

species reactivity

mouse

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

isotype

IgG1κ

NCBI accession no.

NM_001078.2, NM_080682.1

UniProt accession no.

P19320

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... L1CAM(3897), VCAM1(7412)

Application

Anti-VCAM-1 Antibody, clone M/K is an antibody against VCAM-1 for use in FC, IH & IP.
Studies of cell-cell interaction

Cell adhesion assays

Inhibition of lymphopoieisis in Whitlock-Witte long-term marrow cultures

Immunoprecipitation of the VCAM-1 integrin complex

Detection of VCAM-1 cells by immunofluorescence and immunohistochemical staining: acetone or fresh frozen sections work best; although zinc formalin or PLP fixations are reported to work but they have not be verified by Chemicon.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes Murine vascular adhesion molecule (VCAM-1), single chain glycoprotein of Mr 100- kDa (under reducing conditions). VCAM-1 is present on the +/+ 2.4 stromal cell clone, large cells in bone marrow, and a subset of thymic macrophages. The stromal cell antigen is also detectable on the EOMA and BMS2 endothelial cell lines. The antibody reacts with all known isoforms of murine VCAM-1.

Physical form

Format: Purified

Analysis Note

Control
EOMA and BMS2 endothelial cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MAB2242

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Storage Class

12 - Non Combustible Liquids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

CBL1300:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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