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ABS2252-100UL Anti-ZnT8

Anti-ZnT8, Cat. No. ABS2252, is a rabbit polyclonal antibody that detects Zinc transporter 8 (ZnT8) and is tested in Immunofluorescence.

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ABS2252-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABS2252-100UL
      DescriptionAnti-ZnT8
      Alternate Names
      • Zinc transporter 8
      • Solute carrier family 30 member 8
      Background InformationZinc transporter 8 (UniProt: Q8IWU4; also known as ZnT-8, Solute carrier family 30 member 8) is encoded by the SLC30A8 (also known as ZNT8) gene (Gene ID: 169026) in human. Zinc is required for insulin crystallization, storage, and secretion in the secretory granules of pancreatic β-cells. Delivery of Zn2+ into the insulin-containing granules is largely accomplished by the ZnT8 protein. ZnT8 is a homodimeric, multi-pass membrane protein with four cytoplasmic domains, six transmembrane domains, and three extracellular domains. It serves as a granule-specific Zn2+ transporter that is abundantly expressed in pancreatic islet cells. It contains a histidine-rich region, HXXXXXHNH-motif, in its third cytoplasmic domain, which serves as a ligand for zinc. ZnT8 is expressed in both a and b cells, but not in  cells. It plays a major role in Zn2+ uptake into the secretory granules. During stimulated secretion, Zn2+ is shown to be co-released with other granular content into the extracellular medium.
      Loss of ZnT8 function is reported to reduce total islet Zn2+ content as well as free Zn2+ in the cytosol and granules. Loss of ZnT8 also results in reduced dense core granules and increased granule diameter. Studies have shown that the expression of ZnT8 is significantly reduced in the islets of diabetic murine models. Two isoforms of ZnT8 have been described that are produced by alternative splicing. (Ref.: Ghazvini Zadeh, EH., et al. (2020). Cell Reports 32(2); 107904; Dwivedi, OP., et al. (2019). Nat. Genetics. 51(11); 1596-1606).
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antibody in 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-ZnT8, Cat. No. ABS2252, is a rabbit polyclonal antibody that detects Zinc transporter 8 (ZnT8) and is tested in Immunofluorescence.
      Key Applications
      • Immunofluorescence
      Application NotesTested Applications

      Immunofluorescence Analysis: A representative lot detected ZnT8 in Immunofluorescence applications (Zadeh, EHG., et.al. (2020). Cell Rep. 32(2):107904).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 102 amino acids from the C-terminal, cytoplasmic domain of human zinc transporter 9 (ZnT8).
      EpitopeC-terminus
      ConcentrationPlease refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Zinc transporter 8 (ZnT8). It targets an epitope within the C-terminal, cytoplasmic domain.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SLC30A8
      • ZNT8
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight40.76 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunofluorescence in mouse pancreas frozen formalin fixed sections.

      Immunofluorescence Analysis: A 1:100 dilution of this antibody detected ZnT8 in mouse pancreas frozen formalin fixed sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS2252-100UL 04065266298284