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07-227-I-100UG Anti-p34-Arc/ARPC2

Anti-p34-Arc/ARPC2 , Cat. No. 07-227-I, is a rabbit polyclonal antibody that detects Actin-related protein 2/3 complex subunit 2 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

07-227-I-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue Number07-227-I-100UG
      DescriptionAnti-p34-Arc/ARPC2
      Alternate Names
      • Actin-related protein 2/3 complex subunit 2
      • Arp2/3 complex 34 kDa subunit
      • p34-ARC
      Background InformationActin-related protein 2/3 complex subunit 2 (UniProt: O15144; also known as Arp2/3 complex 34 kDa subunit, p34-ARC, ARPC2) is encoded by the ARPC2 (also known as ARC34, PRO2446) gene (Gene ID: 10109) in human. ARPC2 is an evolutionarily conserved actin-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). It holds a central structural position and helps relay both signals and conformational changes. ARPC2 also participates in promoting smooth muscle cell migration. The Arp2/3 complex is shown to mediate the formation of branched actin networks in the cytoplasm, which provides the force for cell motility. This complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription. It is also involved in promoting homologous recombination repair in response to DNA damage. ARPC2 is shown to increase the proliferation and invasion in MKN-28, human gastric cancer, cells. The expression of ARPC2 has been linked with aggressiveness gastric cancer that includes large tumor size, lymph node invasion, high tumor stage, and poor prognosis. (Ref.: Zhang, J., et al. (2017). Mediators of Inflammation. 217; Article ID 5432818).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-p34-Arc/ARPC2 , Cat. No. 07-227-I, is a rabbit polyclonal antibody that detects Actin-related protein 2/3 complex subunit 2 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected p34-Arc/ARPC2 in HeLa, HEK293, and Jurkat cell lysate.

      Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected p34-Arc/ARPC2 in HeLa cell line.

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected p34-Arc/ARPC2 in human colon cancer and human tonsil tissue sections.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of human Actin-related protein 2/3 complex subunit 2 (p34-ARC).
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityAnti-Anti-p34-Arc\ARPC2 is a rabbit polyclonal antibody that detects Actin-related protein 2/3 complex subunit 2. It targets an epitope with in 14 amino acids from the C-terminal region.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine, Mouse, and Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ARPC2
      • ARC34
      • PRO2446
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~34 kDa observed; 34.33 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected p34-Arc/ARPC2 in A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-227-I-100UG 04054839007163