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05-1336

Anti-phospho-Histone H3 (Ser10) Antibody, clone CMA312

clone CMA312, from mouse

Synonym(s):

H3S10P, Histone H3 (phospho S10), H3 histone, family 3A

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
CMA312, monoclonal
Species reactivity:
vertebrates, human
Application:
ChIP, ELISA, ICC, WB, multiplexing
Citations:
20
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biological source

mouse

Quality Segment

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

CMA312, monoclonal

species reactivity

vertebrates, human

technique(s)

ChIP: suitable, ELISA: suitable, immunocytochemistry: suitable, multiplexing: suitable, western blot: suitable

isotype

IgG1

NCBI accession no.

NP_003484.1

UniProt accession no.

P84243

shipped in

wet ice

target post-translational modification

phosphorylation (pSer10)

Gene Information

human ... H3C1(8350)

General description

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming the nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. Phosphorylation at Ser10, which is linked to gene activation, prevents methylation at Lys9 but facilitates acetylation of H3 and H4. Phosphorylated Ser10 is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition, Ser10 phosphorylation is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at Ser10 by Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.
Anti-phospho-Histone H3 (Ser10) Antibody, clone CMA312 is a mouse monoclonal antibody for detection of phospho-Histone H3 (Ser10) also known as H3S10P, Histone H3 (phospho S10) & has been validated in WB, ELISA, ICC, Multiplexing and ChIP.
~17 kDa

Application

ELISA: This antibody has been shown by an outside laboratory to be suitable for ELISA.1
Immunocytochemistry: This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1
Multiplexing:
This antibody specifically recognizes histone H3 phosphorylated on Ser10 by Luminex® assay.

Biochem/physiol Actions

Broad species cross-reactivity is expected, based on sequence homology.

Physical form

Format: Purified

Analysis Note

Western Blot Analysis: 1 μg/mL of this antibody detected phospho-Histone H3 (Ser10) on 1 μg of colcemid-treated HeLa acid extract.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1093

Legal Information

Luminex is a registered trademark of Luminex Corp

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wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Storage Class

12 - Non Combustible Liquids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

05-1336:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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