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MABN2415 Anti-phospho-NHE3 (Ser552) Antibody, clone 14D5

Anti-phospho-NHE3 (Ser552), clone 14D5, Cat. No. MABN2415, is a highly specific mouse monoclonal antibody that targets Sodium/hydrogen exchanger 3 and has been tested for use in ELISA, Immunofluorescence, and Western Blotting.

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MABN2415
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2415
      DescriptionAnti-phospho-NHE3 (Ser552) Antibody, clone 14D5
      Alternate Names
      • Sodium/hydrogen exchanger 3
      • Na(+)/H(+) exchanger 3
      • Solute carrier family 9 member 3
      • NHE-3
      Background InformationSodium/hydrogen exchanger 3 (UniProt: P26433; also known as Na(+)/H(+) exchanger 3, NHE-3, Solute carrier family 9 member 3) is encoded by the Slc9a3 (Nhe3) gene (Gene ID: 24784) in rat. NHE-3 is a multi-pass membrane protein that is involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. It is a major proton extruding system driven by the inward sodium ion chemical gradient. NHE-3 is shown to be most abundant in colon and small intestine and is also present in stomach and in proximal tubes and outer medulla of kidney. In kidneys it mediates the majority of sodium bicarbonate reabsorption by the proximal tubules. NHE-3 can be phosphorylated and regulated by multiple kinases, including PKA, SGK1, and CK2 and its phosphorylation is critical in its regulation. Parathyroid hormone is shown to enhance PKA-mediated phosphorylation of Serine 552, however, this phosphorylation does not affect the activity of NHE-3. (Ref.: Kocinsky, HS., et al. (2007). Am J Physiol Renal Physiol 293 (1); F212-F218).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho-NHE3 (Ser552), clone 14D5, Cat. No. MABN2415, is a highly specific mouse monoclonal antibody that targets Sodium/hydrogen exchanger 3 and has been tested for use in ELISA, Immunofluorescence, and Western Blotting.
      Key Applications
      • ELISA
      • Immunofluorescence
      • Western Blotting
      Application NotesImmunofluorescence Analysis: A representative lot detected phospho-NHE3 (Ser552) in Immunofluorescence applications (Kocinsky, H.S., et. al. (2005). Am J Physiol Renal Physiol. 289(2):F249-58).

      Western Blotting Analysis: A representative lot detected phospho-NHE3 (Ser552) in Western Blotting applications (Kocinsky, H.S., et. al. (2005). Am J Physiol Renal Physiol. 289(2):F249-58; Kocinsky, H.S., et. al. (2007). Am J Physiol Renal Physiol. 293(1):F212-8).

      ELISA Analysis: A representative lot detected phospho-NHE3 (Ser552) in ELISA applications (Kocinsky, H.S., et. al. (2005). Am J Physiol Renal Physiol. 289(2):F249-58).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 22 amino acids surrounding phospho Serine 552 from the C-terminal half of rat Sodium/hydrogen exchanger 3 (NHE3).
      Clone14D5
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityThis mouse monoclonal antibody (clone 14D5) specifically detects Sodium/hydrogen exchanger 3 phosphorylated on Serine 552.
      IsotypeIgG2aκ
      Species Reactivity
      • Rat
      • Opossum
      • Mouse
      • Bovine
      • Human
      Species Reactivity NoteMouse, Bovine, Human, Opossum, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Slc9a3
      • Nhe3
      Modifications
      • Phosphorylation
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~85 kDa observed; 93.11 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in COS-7 cells either transfected with wild type NHE3, mutated NHE3 (S552A), or untransfected COS-7 (WT) cell lysates.

      Western Blotting Analysis: 2 µg/mL of this antibody detected Sodium/hydrogen exchanger 3 in lysate from COS-7 cells transfected with wild- type NHE-3 (pSer552). It did not detect NHE-3 in lysate from untransfected COS-7 cells or those transfected with mutated NHE-3 (S552A).


      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2415 04054839410475

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