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ABS2246-25UG Anti-phospho PKLR (Ser113)

Anti-phospho PKLR (Ser113), Cat. No. ABS2246, is a rabbit polyclonal antibody that detects Pyruvate kinase PKLR phosphorylated on serine 113 and is tested for use in Immunofluorescence and Western Blotting.

ABS2246-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABS2246-25UG
      DescriptionAnti-phospho PKLR (Ser113)
      Alternate Names
      • Pyruvate kinase PKLR
      • EC:2.7.1.40
      • L-PK
      • Pyruvate kinase isozymes L
      Background InformationPyruvate kinase PKLR (UniProt: P12928-2; also known as EC:2.7.1.40, L-PK, Pyruvate kinase isozymes L/R) is encoded by the Pklr gene (Gene ID: 24651) in rat. Pyruvate kinas (PK) is a key glycolytic enzyme that catalyzes the hydrolysis of phosphoenolpyruvate (PEP) to pyruvate, generating ATP in this process. Four tissue specific isozymes of PK have been described in mammals: L (liver), R (erythrocytes), M1 (muscle) and ubiquitous M2. Except for M1 isozyme all other isozymes display positive cooperative kinetic behavior upon binding the allosteric activator fructose-1,6-diphosphate and in the presence of increasing concentrations of PEP. Phosphorylation of PK liver isozyme at serine 113 has been correlated with insulin resistance in rats fed a high-fat diet. Mice fed a high fat diet exhibit a significantly increased level of phospho-Ser 113 form, which declines significantly upon treatment with a pyruvate kinase activator. It has been shown that in rats fed a high-fat diet CDKs are activated and cause phosphorylation of serine 113. The amino acid sequence surrounding pS113 site is shown to have features of a CDK substrate. Phosphorylation of liver PK at serine 113 results in its retention in the nucleus and inhibition of CDK can reduce its nuclear retention and cause an increase in cytosolic enzyme activity. (Ref.: Abulizi, A., et al. (2020). Cell Metab. 32(5); 751-766; Gassaway, BM., et al. (2019). Cell Rep. 29(11); 3394-3404).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-phospho PKLR (Ser113), Cat. No. ABS2246, is a rabbit polyclonal antibody that detects Pyruvate kinase PKLR phosphorylated on serine 113 and is tested for use in Immunofluorescence and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesWestern Blotting Analysis (WB): A representative lot of this antibody detected Full-length phosphorylated PKL pS113 in Western Blotting applications. (Courtesy of Dr. Jesse Rinehart Lab, Yale University).

      Western Blotting Analysis: A representative lot detected Pyruvate kinase PKLR in Western Blotting applications. (Gassaway, B.M., et.al, (2019). Cell Rep. 29(11):3394-3404).

      Immunofluorescence Analysis: A representative lot detected Pyruvate kinase PKLR in Immunofluorescence applications (Gassaway, B.M., et.al, (2019). Cell Rep. 29(11):3394-3404).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 12 amino acids surrounding the phosphoserine 113 from the N-terminal region of rat Pyruvate. kinase PKLR.
      EpitopeN-terminal
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects Pyruvate Kinase PKLR phosphorylated on serine 113.
      IsotypeIgG
      Species Reactivity
      • Rat
      Species Reactivity NoteRat. Predicted to react with Human, Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Pklr
      Modifications
      • Phosphorylation
      Purification MethodProtein A purified
      UniProt Number
      Molecular Weight~58 kDa observed; 58.79 kDa calculated for L-isoform. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting on Full-length phosphorylated PKL pS113.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected full-length rat Pyruvate kinase (PKLR) phosphorylated on serine 113, but not the unphosphorylated PKLR
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS2246-25UG 04065265324397