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05-1059

Anti-phospho-p38α(Thr180/Tyr182) Antibody, clone 8.78.8, rabbit monoclonal

culture supernatant, clone 8.78.8, from rabbit

Synonym(s):

CSAID-binding protein, Csaids binding protein, Cytokine suppressive anti-inflammatory drug-binding protein, MAP kinase MXI2, MAP kinase p38 alpha, MAX-interacting protein 2, Mitogen-activated protein kinase p38 alpha, cytokine suppressive anti-inflammato

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
8.78.8, monoclonal
Species reactivity:
human, vertebrates, mouse
Application:
IF, WB
Citations:
4
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biological source

rabbit

Quality Segment

100

antibody form

culture supernatant

antibody product type

primary antibodies

clone

8.78.8, monoclonal

species reactivity

human, vertebrates, mouse

technique(s)

immunofluorescence: suitable, western blot: suitable

isotype

IgG

NCBI accession no.

NP_001306

UniProt accession no.

Q15759

shipped in

wet ice

target post-translational modification

phosphorylation (pThr180/pTyr182)

Gene Information

human ... MAPK14(1432)

General description

38 kDa
The p38 family refers to the SAPK2/3 Kinases (Stess-Activated Protein Kinases), a subfamily of JNK/SAPK family. p38/SAPK Kinases are also activated by stresses, most notably inflammatory cytokines, irradiation, and certain toxins such as anisomycin and arsenite. SAPKs have been implicated in many pathological conditions, including inflammation, cancer and neurodegenerative diseases. As with the other MAPK family members (ERK, JNK, p38MAPK, and ERK5), p38 is activated through phosphorylation on both threonine and tyrosine residues at the Thr-X-Tyr dual phosphorylation motif, where X is Gly for p38. Once phosphorylated and active, it then goes on to phosphorylate downstream targets in both the cytoplasm and the nucleus. Some of their main targets of p38 are the kinase MAPKAPK2 and the transcription factor ATF2 that are activated via their phosphorylation.

Immunogen

Epitope: Phosphorylated Thr180/Tyr182
Synthetic peptide corresponding to amino acids surrounding and including phosphorylated Thr180/Tyr182 of human p38α.

Application

Anti-phospho-p38α(Thr180/Tyr182) Antibody, clone 8.78.8 detects level of phospho-p38α(Thr180/Tyr182) & has been published & validated for use in WB & IF.
Research Sub Category
MAP Kinases

Inflammation & Autoimmune Mechanisms

Biochem/physiol Actions

Predicted to cross-react with many other species based on 100% sequence homology with immunogen.
This antibody detects p38α when phosphorylated on Thr180/Tyr182.

Physical form

Rabbit monoclonal antibody in culture supernatant containing 0.05% sodium azide.

Analysis Note

Evaluated by western blot on anisomycin-treated HeLa cell lysate.
Western Blot Analysis: 1:5,000-1:10,000 dilution of this antibody was used to detect p38 phosphorylated on Thr180/Tyr182 in anisomycin-treated HeLa and NIH/3T3 cell lysate.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

05-1059:

jan

Certificates of Analysis (COA)

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