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MABN2678-25UG Anti-phospho-α-synuclein (Ser129) Antibody, clone PS129

Anti-phospho- -synuclein (Ser129), clone PS129, Cat. No. MABN2678, is a mouse monoclonal antibody that detects -synuclein and is tested for use in Western Blotting, Immunohistochemistry, and ELISA.

MABN2678-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2678-25UG
      DescriptionAnti-phospho-α-synuclein (Ser129) Antibody, clone PS129
      Alternate Names
      • Non-A beta component of AD amyloid
      • Non-A4 component of amyloid precursor (NACP)
      Background InformationAlpha-synuclein (UniProt: P37840; also known as Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP) is encoded by the SNCA (also known as NACP, PARK1) gene (Gene ID: 6622) in human. Alpha-synuclein is a membrane-bound protein in dopaminergic neurons that is involved in the regulation of dopamine release and transport. It is also reported to induce fibrillization of microtubule-associated protein tau and reduces neuronal responsiveness to various apoptotic stimuli. It is primarily expressed in the brain and is associated with the regulation of neurotransmitter release at synapses. Alpha-synuclein is also found in the cytoplasm, where it exists in a dynamic equilibrium between a soluble cytosolic form and a membrane-bound form. It associates with lipid membranes, particularly those of synaptic vesicles. Aggregation of alpha-synuclein is known to be a causative factor in the development of Parkinson's disease (PD), which leads to the degeneration of dopaminergic neurons in the substantia nigra (SN) in the midbrain region. -synuclein can be phosphorylated, predominantly on serine residues by casein kinase 1 (CK1). Phosphorylation by CK1 appears to occur on residue (serine 129) distinct from the residue (tyrosine 125) phosphorylated by other kinase FYN. The phosphorylation of -synuclein at serine 129 (pS129) has been extensively studied due to its significant implications in neurodegenerative diseases, particularly Parkinson's disease (PD) and other synucleinopathies. Phosphorylation of Ser129 is reported to be selective and extensive in synucleinopathy lesions and promotes insoluble fibril formation. This serves as a potential biomarker for disease diagnosis and progression and offers a promising target for therapeutic intervention. (Ref.: Vaikath, N.N., et al. (2015). Neurobiol Dis. 79:81-99; Majbour, N.K., et al. (2016). Mol Neurodegener. 19:11:7; El-Agnaf, O., et al. (2017). Neurobiol Dis. 104:85-96).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-phospho- -synuclein (Ser129), clone PS129, Cat. No. MABN2678, is a mouse monoclonal antibody that detects -synuclein and is tested for use in Western Blotting, Immunohistochemistry, and ELISA.
      Key Applications
      • Western Blotting
      • ELISA
      • Immunohistochemistry
      Application NotesTested Applications

      ELISA Analysis: A representative lot detected -synuclein in ELISA application (Majbour, N.K., et al. (2016). Mol Neurodegener. 11:7; El-Agnaf, O., et al. (2017). Neurobiol Dis. 104: 85-96).

      Immunohistochemistry Application: A representative lot detected phospho- -synuclein (Ser129) in Immunohistochemistry application (Vaikath, N.N., et al. (2015). Neurobiol Dis. 79:81-99).

      Western Blotting Analysis: A representative lot detected phospho- -synuclein (Ser129) in Western Blotting application (Majbour, N.K., et al. (2016). Mol Neurodegener. 11:7).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 9 amino acids surrounding phosphoserine 129 from the C-terminal region of human -synuclein.
      EpitopeC-terminal
      ClonePS129
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone PS129 is a mouse monoclonal antibody that specifically detects -synuclein phosphorylated on serine 129.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse. Predicted to react with Rat, Bovine, Porcine based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SNCA
      Modifications
      • Phosphorylation
      Purification MethodProtein G purified
      UniProt Number
      Molecular WeightTarget molecular weight ~16 kDa observed; 14.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant -synuclein phosphorylated S129 and recombinant -synuclein monomers.

      Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected -synuclein phosphorylated on serine 129 in recombinant -synuclein phosphorylated S129 protein, but not in recombinant -synuclein monomers.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2678-25UG 04065272161480